Effect of combined long-term starvation and γ-irradiation on membrane fatty acids and cell surface hydrophobicity of Salmonella enterica serovar Typhimurium

This study was carried out to explore the adaptive mechanisms of Salmonella enterica serovar Typhimurium, in particular the implication of fatty acids (FA) in the remodeling of membrane lipid composition to overcome the combined effects of long-term starvation and γ-irradiation stresses. In addition...

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Bibliographic Details
Published in:Journal of dairy science 2015-12, Vol.98 (12), p.8525-8530
Main Authors: Lagha, Rihab, Ben Abdallah, Fethi, Masmoudi, Ahmed Salaheddine
Format: Article
Language:English
Subjects:
Animals
Cell Membrane - chemistry
Chromatography, Gas
fatty acids
Fatty Acids - analysis
Gamma Rays
Hydrophobic and Hydrophilic Interactions
hydrophobicity
long-term starvation
Membrane Lipids - analysis
Membrane Lipids - physiology
Membrane Lipids - radiation effects
Salmonella Typhimurium
Salmonella typhimurium - chemistry
Salmonella typhimurium - physiology
Salmonella typhimurium - radiation effects
Serogroup
γ-irradiation
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Summary:This study was carried out to explore the adaptive mechanisms of Salmonella enterica serovar Typhimurium, in particular the implication of fatty acids (FA) in the remodeling of membrane lipid composition to overcome the combined effects of long-term starvation and γ-irradiation stresses. In addition, cell surface hydrophobicity was also evaluated. The bacterial strains (control and starved) were treated with a nonlethal γ-irradiation dose of 0.5 kGy and sublethal doses of 1 kGy. Gas chromatography analysis showed that the FA composition of starved and γ-irradiated cells was modified. However starvation combined with γ-irradiation induced more modifications in the FA composition than γ-irradiation or starvation alone. Indeed, the unsaturated FA-to-saturated FA ratio decreased significantly for both strains compared with γ-irradiated cells, as main consequence of the cyclic FA formation. Our results showed that starvation, irradiation, or combined stresses significantly influenced the hydrophobicity, and this may have affected the virulence state of Salmonella Typhimurium cells. This study represents one of the few to demonstrate the modifications on bacterial membrane as a cellular response to survive to the ionizing radiation combined with long-term starvation stress.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2015-9984