Erythrocyte Susceptibility to Oxidative Stress in Chronic Renal Failure Patients Under Different Substitutive Treatments

:  An increased oxidative stress is now considered one of the major risk factors in chronic renal failure (CRF) patients that may be exacerbated by dialysis. It has been postulated that this increased oxidative stress might cause an augmented red blood cell (RBC) membrane lipid peroxidation with the...

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Bibliographic Details
Published in:Artificial organs 2005-01, Vol.29 (1), p.67-72
Main Authors: Lucchi, Leonardo, Bergamini, Stefania, Iannone, Anna, Perrone, Salvatore, Stipo, Lucia, Olmeda, Fabio, Caruso, Francesco, Tomasi, Aldo, Albertazzi, Alberto
Format: Article
Language:English
Subjects:
Case-Control Studies
Catalase - antagonists & inhibitors
Chromatography, High Pressure Liquid
Chronic  renal failure
Enzyme Inhibitors - pharmacology
Erythrocytes - metabolism
Glutathione
Glutathione - metabolism
Hemodialysis
Humans
Kidney Failure, Chronic - metabolism
Kidney Failure, Chronic - therapy
Kidney Transplantation
Malondialdehyde - metabolism
Middle Aged
Oxidative stress
Oxidative Stress - physiology
Peritoneal dialysis
Renal Dialysis - methods
Sodium Azide - pharmacology
tert-Butylhydroperoxide - pharmacology
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Summary::  An increased oxidative stress is now considered one of the major risk factors in chronic renal failure (CRF) patients that may be exacerbated by dialysis. It has been postulated that this increased oxidative stress might cause an augmented red blood cell (RBC) membrane lipid peroxidation with the consequent alteration in membrane deformability. The aim of this study was to evaluate RBC susceptibility to an in vitro induced oxidative stress and RBC antioxidant potential in different groups of CRF patients undergoing different substitutive treatment modalities. Fifteen end‐stage CRF patients were evaluated in conservative treatment, 23 hemodialysis (HD) patients, 15 continuous ambulatory peritoneal dialysis (CAPD) patients, 15 kidney transplanted patients, and 16 controls. Their RBCs were incubated with the oxidative stress‐inducing agent tert‐butylhydroperoxide both in the presence and in the absence of the catalase inhibitor sodium azide, and the level of malondialdehyde (MDA) (a product of lipid peroxidation), was measured at 0, 5, 10, 15, and 30 min of incubation. In addition, the RBC content of reduced glutathione (GSH) was measured by HPLC. As opposed to the controls, RBCs from end‐stage CRF patients exhibited an increased sensitivity to oxidative stress induced in vitro, both in the absence and presence of a catalase inhibitor, as demonstrated by a significantly higher level of MDA production at all the incubation times (P 
ISSN:0160-564X
1525-1594
DOI:10.1111/j.1525-1594.2004.29011.x