PsAP2 an AP2/ERF family transcription factor from Papaver somniferum enhances abiotic and biotic stress tolerance in transgenic tobacco

The AP2/ERFs are one of the most important family of transcription factors which regulate multiple responses like stress, metabolism and development in plants. We isolated PsAP2 a novel AP2/ERF from Papaver somniferum which was highly upregulated in response to wounding followed by ethylene, methyl...

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Bibliographic Details
Published in:Plant molecular biology 2015-09, Vol.89 (1-2), p.173-186
Main Authors: Mishra, Sonal, Phukan, Ujjal J, Tripathi, Vineeta, Singh, Dhananjay K, Luqman, Suaib, Shukla, Rakesh Kumar
Format: Article
Language:English
Subjects:
Abiotic stress
abscisic acid
Amino Acid Sequence
Base Sequence
Biochemistry
Biomedical and Life Sciences
biotic stress
ethylene
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
gene overexpression
Gene Silencing - physiology
inositol-3-phosphate synthase
Life Sciences
Metabolism
methyl jasmonate
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - physiology
Oxygen
Papaver - genetics
Papaver - physiology
Papaver somniferum
Phylogeny
Plant biology
Plant Pathology
Plant Proteins - genetics
Plant Proteins - physiology
Plant Sciences
Plants, Genetically Modified - genetics
Plants, Genetically Modified - physiology
Promoter Regions, Genetic - genetics
Promoter Regions, Genetic - physiology
reporter genes
stress tolerance
Stress, Physiological - genetics
Stress, Physiological - physiology
Tobacco
transcription (genetics)
transcription factors
Transcription Factors - genetics
Transcription Factors - physiology
transcriptomics
transgenic plants
yeasts
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Summary:The AP2/ERFs are one of the most important family of transcription factors which regulate multiple responses like stress, metabolism and development in plants. We isolated PsAP2 a novel AP2/ERF from Papaver somniferum which was highly upregulated in response to wounding followed by ethylene, methyl jasmonate and ABA treatment. PsAP2 showed specific binding with both DRE and GCC box elements and it was able to transactivate the reporter genes in yeast. PsAP2 overexpressing transgenic tobacco plants exhibited enhanced tolerance towards both abiotic and biotic stresses . Real time transcript expression analysis showed constitutive upregulation of tobacco Alternative oxidase1a and Myo-inositol-1-phosphate synthase in PsAP2 overexpressing tobacco plants. Further, PsAP2 showed interaction with NtAOX1a promoter in vitro, it also specifically activated the NtAOX1a promoter in yeast and tobacco BY2 cells. The silencing of PsAP2 using VIGS lead to significant reduction in the AOX1 level in P. somniferum. Taken together PsAP2 can directly bind and transcriptionally activate NtAOX1a and its overexpression in tobacco imparted increased tolerance towards both abiotic and biotic stress.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-015-0361-7