Structures of three different neutral polysaccharides of Acinetobacter baumannii, NIPH190, NIPH201, and NIPH615, assigned to K30, K45, and K48 capsule types, respectively, based on capsule biosynthesis gene clusters

•Neutral capsular polysaccharides were isolated from three Acinetobacter baumannii strains.•Structures of the polysaccharides were established by NMR and chemical approaches.•Capsule biosynthesis gene clusters were analyzed and found to match the structures established.•Glycosyltransferases encoded...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate research 2015-11, Vol.417, p.81-88
Main Authors: Shashkov, Alexander S., Kenyon, Johanna J., Arbatsky, Nikolay P., Shneider, Mikhail M., Popova, Anastasiya V., Miroshnikov, Konstantin A., Volozhantsev, Nikolay V., Knirel, Yuriy A.
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Acinetobacter baumannii - chemistry
Acinetobacter baumannii - genetics
Acinetobacter baumannii - isolation & purification
Acinetobacter baumannii - metabolism
Acinetobacter Infections - microbiology
Antigens, Bacterial - chemistry
Bacterial Capsules - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Capsular polysaccharide structure
Carbohydrate Sequence
Gene Expression
Genetic Loci
Glycosyltransferase
Glycosyltransferases - genetics
Glycosyltransferases - metabolism
Humans
K locus
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Multigene Family
Polysaccharide gene cluster
Polysaccharides, Bacterial - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Neutral capsular polysaccharides were isolated from three Acinetobacter baumannii strains.•Structures of the polysaccharides were established by NMR and chemical approaches.•Capsule biosynthesis gene clusters were analyzed and found to match the structures established.•Glycosyltransferases encoded in the clusters were tentatively assigned to glycosidic linkages. [Display omitted] Neutral capsular polysaccharides (CPSs) were isolated from Acinetobacter baumannii NIPH190, NIPH201, and NIPH615. The CPSs were found to contain common monosaccharides only and to be branched with a side-chain 1→3-linked β-d-glucopyranose residue. Structures of the oligosaccharide repeat units (K units) of the CPSs were elucidated by 1D and 2D 1H and 13C NMR spectroscopy. Novel CPS biosynthesis gene clusters, designated KL30, KL45, and KL48, were found at the K locus in the genome sequences of NIPH190, NIPH201, and NIPH615, respectively. The genetic content of each gene cluster correlated with the structure of the CPS unit established, and therefore, the capsular types of the strains studied were designated as K30, K45, and K48, respectively. The initiating sugar of each K unit was predicted, and glycosyltransferases encoded by each gene cluster were assigned to the formation of the linkages between sugars in the corresponding K unit.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2015.09.004