bacterial haloalkane dehalogenase gene as a negative selectable marker in Arabidopsis

The dhlA gene of Xanthobacter autotrophicus GJ10 encodes a dehalogenase which hydrolyzes dihaloalkanes, such as 1,2-dichloroethane (DCE), to a halogenated alcohol and an inorganic halide (Janssen et al., 1994, Annu. Rev. Microbiol. 48, 163-191). In Xanthobacter, these alcohols are further catabolize...

Full description

Saved in:
Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 1999-06, Vol.18 (5), p.571-576
Main Authors: Naested, H, Fennema, M, Hao, L, Andersen, M, Janssen, D.B, Mundy, J
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Arabidopsis - genetics
Arabidopsis thaliana
bacteria
biochemical pathways
Biodegradation of pollutants
Biological and medical sciences
biomarkers
Biotechnology
Brassica
Brassica rapa subsp. campestris
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on plants and fungi
Environment and pollution
enzyme activity
Ethylene Dibromide - metabolism
ethylene dichloride
Ethylene Dichlorides - metabolism
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic Markers
Gram-Negative Aerobic Rods and Cocci - genetics
hydrolases
Hydrolases - biosynthesis
Hydrolases - genetics
in vitro selection
Industrial applications and implications. Economical aspects
metabolism
metabolites
Nicotiana
Nicotiana tabacum
Oryza
Oryza sativa
Plants, Genetically Modified
Selection, Genetic
toxicity
Xanthobacter
Zea mays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The dhlA gene of Xanthobacter autotrophicus GJ10 encodes a dehalogenase which hydrolyzes dihaloalkanes, such as 1,2-dichloroethane (DCE), to a halogenated alcohol and an inorganic halide (Janssen et al., 1994, Annu. Rev. Microbiol. 48, 163-191). In Xanthobacter, these alcohols are further catabolized by alcohol and aldehyde dehydrogenase activities, and by the product of the dhlB gene to a second halide and a hydroxyacid. The intermediate halogenated alcohols and, in particular, the aldehydes are more toxic than the haloalkane substrates or the pathway products. We show here that plants, including Arabidopsis, tobacco, oil seed rape and rice, do not express detectable haloalkane dehalogenase activities, and that wild-type Arabidopsis grows in the presence of DCE. In contrast, DCE applied as a volatile can be used to select on plates or in soil transgenic Arabidopsis which express dhlA. The dhlA marker therefore provides haloalkane dehalogenase reporter activity and substrate dependent negative selection in transgenic plants.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313X.1999.00477.x