Thiazolidinediones Down-Regulate Plasminogen Activator Inhibitor Type 1 Expression in Human Vascular Endothelial Cells: A Possible Role for PPARγ in Endothelial Function

The effect of peroxisome proliferator-activated receptor (PPAR) γ activators, thiazolidinediones, on plasminogen activator type 1 (PAI-1) was examined in cultured human umbilical vein endothelial cells (HUVEC). Tumor necrosis factor α (TNF-α) enhanced PAI-1 secretion and mRNA expression by approxima...

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Published in:Biochemical and biophysical research communications 1999-05, Vol.258 (2), p.431-435
Main Authors: Kato, Ken, Satoh, Hiroaki, Endo, Yuichi, Yamada, Daishiro, Midorikawa, Sanae, Sato, Wakano, Mizuno, Kenji, Fujita, Teizo, Tsukamoto, Kazuhisa, Watanabe, Tsuyoshi
Format: Article
Language:English
Subjects:
human umbilical vein endothelial cell
peroxisome proliferator-activated receptor γ
plasminogen activator inhibitor type 1
thiazolidinediones
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Summary:The effect of peroxisome proliferator-activated receptor (PPAR) γ activators, thiazolidinediones, on plasminogen activator type 1 (PAI-1) was examined in cultured human umbilical vein endothelial cells (HUVEC). Tumor necrosis factor α (TNF-α) enhanced PAI-1 secretion and mRNA expression by approximately 2-fold. The thiazolidinediones, troglitazone and pioglitazone, decreased basal and TNF-α-stimulated PAI-1 secretion and mRNA expression in HUVEC in a dose-dependent fashion. PPARγ mRNA in HUVEC could be detected by reverse transcriptase-polymerase chain reaction using specific primers. These results suggest that PPARγ may regulate PAI-1 expression in HUVEC and that thiazolidinediones have a therapeutic potential for improving endothelial dysfunction observed in insulin resistance.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0648