Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum β-lactamase producers and sensitive strains

Background Escherichia coli is a frequent causative agent of urinary tract infections, and increasing resistance of E. coli to antimicrobials presents a growing challenge. Methods Here we compare phenotypes of extended-spectrum β-lactamase (ESBL) producers ( n  = 220) with a control group of sensiti...

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Bibliographic Details
Published in:Journal of microbiology, immunology and infection immunology and infection, 2015-06, Vol.48 (3), p.329-334
Main Authors: Siskova, Petra, Cernohorska, Lenka, Mahelova, Martina, Turkova, Kristyna, Woznicova, Vladana
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents - pharmacology
Bacterial Typing Techniques
beta-Lactamases - secretion
biochemistry
Biofilms - growth & development
Cell Wall - chemistry
Ciprofloxacin - pharmacology
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli - physiology
Escherichia coli Infections - microbiology
extended-spectrum β-lactamase
Female
Genetic Variation
Genotype
Hemolysis
Humans
Infectious Disease
Male
Medical Education
Microbial Sensitivity Tests
Middle Aged
O Antigens - analysis
Ornithine - analysis
Phenotype
Urinary Tract Infections - microbiology
Urine - microbiology
Young Adult
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Summary:Background Escherichia coli is a frequent causative agent of urinary tract infections, and increasing resistance of E. coli to antimicrobials presents a growing challenge. Methods Here we compare phenotypes of extended-spectrum β-lactamase (ESBL) producers ( n  = 220) with a control group of sensitive strains (non-ESBL producers; n  = 150). For each strain, we assessed the presence of O25 antigen, hemolysis, biofilm production, sensitivity to antibiotics, and biochemical profile. Results Compared to the control group, ESBL producers were more frequently O25 positive (6.0% vs. 42.3%) and less frequently hemolytic (34.7% vs. 6.4%). Comparison of biofilm production in brain–heart infusion (BHI) and in BHI with 4% glucose supplementation showed that ESBL-positive strains produced biofilm in BHI with glucose less intensely than the control group ( p  
ISSN:1684-1182
1995-9133
DOI:10.1016/j.jmii.2014.04.010