photoperiod-insensitive barley line contains a light-labile phytochrome B

Barley (Hordeum vulgare L.) is a long-day plant whose flowering is enhanced when the photoperiod is supplemented with far-red light, and this promotion is mediated by phytochrome. A chemically mutagenized dwarf cultivar of barley was selected for early flowering time (barley maturity daylength respo...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1999-03, Vol.119 (3), p.1033-1039
Main Authors: Hanumappa, M, Pratt, L.H, Cordonnier-Pratt, M.M, Deitzer, G.F
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Avena sativa
Barley
Biological and medical sciences
Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation
Coleoptiles
cultivars
dark
dwarfing
Economic plant physiology
far-red light
Flowering
Flowering, floral biology, reproduction patterns
Fundamental and applied biological sciences. Psychology
Genes
Genetic loci
Genotypes
Growth and Development
height
Hordeum vulgare
immunohistochemistry
inhibition
leaves
length
light
mutants
Photoperiod
phytochrome
plant characteristics
Plant physiology and development
Plants
Seedlings
Seeds
stability
tillering
Vegetative and sexual reproduction, floral biology, fructification
width
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Summary:Barley (Hordeum vulgare L.) is a long-day plant whose flowering is enhanced when the photoperiod is supplemented with far-red light, and this promotion is mediated by phytochrome. A chemically mutagenized dwarf cultivar of barley was selected for early flowering time (barley maturity daylength response [BMDR]-1) and was made isogenic with the cultivar Shabet (BMDR-8) by backcrossing. BMDR-1 was found to contain higher levels of both phytochrome A and phytochrome B in the dark on immunoblots with monoclonal antibodies from oat (Avena sativa L.) that are specific to different members of the phytochrome gene family. Phytochrome A was light labile in both BMDR-1 and BMDR-8, decreasing to very low levels after 4 d of growth in the light. Phytochrome B was light stable in BMDR-8, being equal in both light and darkness. However, phytochrome B became light labile in BMDR-1 and this destabilization of phytochrome B appeared to make BMDR-1 insensitive to photoperiod. In addition, both the mutant and the wild type lacked any significant promotion of flowering in response to a pulse of far-red light given at the end of day, and the end-of-day, far-red inhibition of tillering is normal in both, suggesting that phytochrome B is not involved with these responses in barley.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.119.3.1033