novel satellite DNA isolated in Pecten jacobaeus shows high sequence similarity among molluscs

The aim of this work is to investigate the sequence conservation and the evolution of repeated DNA in related species. Satellite DNA is a component of eukaryotic genomes and is made up of tandemly repeated sequences. These sequences are affected by high rates of mutation that lead to the occurrence...

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Bibliographic Details
Published in:Molecular genetics and genomics : MGG 2015-10, Vol.290 (5), p.1717-1725
Main Authors: Petraccioli, Agnese, Odierna, Gaetano, Capriglione, Teresa, Barucca, Marco, Forconi, Mariko, Olmo, Ettore, Biscotti, Maria Assunta
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Base Sequence
Biochemistry
Biomedical and Life Sciences
Blotting, Southern
Chromosomes
DNA, Satellite - genetics
Evolution
Genomes
Genomics
Human Genetics
Hypotheses
In Situ Hybridization, Fluorescence
Life Sciences
Microbial Genetics and Genomics
Molecular Sequence Data
Mollusca
Original Paper
Pecten - genetics
Pecten jacobaeus
Plant Genetics and Genomics
Satellite DNA
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Taxonomy
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Summary:The aim of this work is to investigate the sequence conservation and the evolution of repeated DNA in related species. Satellite DNA is a component of eukaryotic genomes and is made up of tandemly repeated sequences. These sequences are affected by high rates of mutation that lead to the occurrence of species-specific satellite DNAs, which are different in terms of both quantity and quality. In this work, a novel repetitive DNA family, named PjHhaI sat, is described in Pecten jacobaeus. The quantitative analyses revealed a different abundance of this element in the molluscan species investigated in agreement with the “library hypothesis” even if, in this case, at a high taxonomic level. In addition, the qualitative analysis demonstrated an astonishing sequence conservation not only among scallops but also in six other molluscan species belonging to three classes. These findings suggest that the PjHhaI sat may be considered as the most ancients of DNA described so far, which remained “frozen” during molluscan evolution. The widespread distribution of this sat DNA in molluscs as well as its long evolutionary preservation open up questions on the functional role of this element. A future challenge might be the identification of proteins or molecules which interact with the PjHhaI sat.
ISSN:1617-4615
1617-4623
DOI:10.1007/s00438-015-1036-4