Effects of autologous SCF- and G-CSF-mobilized bone marrow stem cells on hypoxia-inducible factor-1 in rats with ischemia-reperfusion renal injury

To explore the mechanism whereby stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) jointly mobilize bone marrow stem cells (BMSCs) and promote kidney repair, male Sprague-Dawley rats were randomly assigned into 4 groups. In the treatment control group, rats were administered S...

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Published in:Genetics and molecular research 2015-04, Vol.14 (2), p.4102-4112
Main Authors: Bi, L Y, Zhao, D A, Yang, D S, Guo, J G, Liang, B, Zhang, R X, Zhao, J L, Bai, H T, Li, S J
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells - metabolism
Erythropoietin - metabolism
Granulocyte Colony-Stimulating Factor - metabolism
Hematopoietic Stem Cells - metabolism
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Kidney - injuries
Kidney - metabolism
Male
Random Allocation
Rats
Rats, Sprague-Dawley
Reperfusion Injury
Stem Cell Factor - metabolism
Vascular Endothelial Growth Factor A - metabolism
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Summary:To explore the mechanism whereby stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) jointly mobilize bone marrow stem cells (BMSCs) and promote kidney repair, male Sprague-Dawley rats were randomly assigned into 4 groups. In the treatment control group, rats were administered SCF (200 μg·kg(-1)·day(-1)) and G-CSF (50 μg·kg-1·day-1) for 5 days. In the treatment group, RIRI models were established, and 6 h later, SCF (200 μg·kg(-1)·day(-1)) and G-CSF (50 μg·kg(-1)·day(-1)) were administered for 5 days. In the model and treatment groups, tubular epithelial cell degeneration and necrosis were noticed, but the extent of repair in the treatment group was significantly better than in the model group. Five days after the operation, renal tissue CD34+ cells significantly increased in the model and treatment groups compared with the control and treatment control groups. HIF-1α, VEGF, and EPO expression in treatment groups increased significantly compared with the other groups. HIF- 1α, VEGF, EPO expression in the treatment control group increased significantly compared with the control group. Joint use of SCF and G-CSF increased the number of BMSCs in damaged kidney tissue and reduced the degree of renal tissue damage. BMSCs promote increased HIF-1α expression in renal tissue. Increased kidney tissue HIF- 1α and its target gene products VEGF and EPO expression possibly induce SCF and G-CSF to promote acute tubular necrosis repair.
ISSN:1676-5680
1676-5680
DOI:10.4238/2015.April.27.25