Effect of curcumin on p38MAPK expression in DSS-induced murine ulcerative colitis

The aim of this study was to determine the therapeutic effect of curcumin on dextran sulfate sodium-induced ulcerative colitis (UC) and to explore the related mechanism. Sixty mice were randomly divided into 6 groups. A group was the normal control group; B group was the model group; C group was the...

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Bibliographic Details
Published in:Genetics and molecular research 2015-01, Vol.14 (2), p.3450-3458
Main Authors: Li, C-P, Li, J-H, He, S-Y, Chen, O, Shi, L
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Colitis, Ulcerative - chemically induced
Colitis, Ulcerative - drug therapy
Colitis, Ulcerative - enzymology
Colon - drug effects
Colon - enzymology
Colon - pathology
Curcumin - pharmacology
Dextran Sulfate
Drug Evaluation, Preclinical
Female
Gene Expression - drug effects
Granulocyte Colony-Stimulating Factor - metabolism
Interleukin-3 - metabolism
Intestinal Mucosa - enzymology
MAP Kinase Signaling System
Mice, Inbred BALB C
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - metabolism
Recombinant Fusion Proteins - metabolism
Tumor Necrosis Factor-alpha - metabolism
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Summary:The aim of this study was to determine the therapeutic effect of curcumin on dextran sulfate sodium-induced ulcerative colitis (UC) and to explore the related mechanism. Sixty mice were randomly divided into 6 groups. A group was the normal control group; B group was the model group; C group was the 1.5 mg/kg dexamethasone group based on the B group; and D, E and F groups were 15, 30, and 60 mg/kg curcumin groups, respectively, based on the B group. The mice were killed 7 days after treatment; the expression of TNF-α and MPO in colon tissue was determined with ELISA, and colon p-p38MAPK and p38MAPK mRNA expression was evaluated by immunohistochemistry and RT-PCR, respectively. In the C, D, E, and F groups, TNF-α and MPO levels significantly decreased (P < 0.05), and the expression of p-p38MAPK also significantly decreased (P < 0.01). The expression of p38MAPK mRNA in the C, D, E, and F groups decreased (P < 0.01), and there was a statistically significant difference between the E and F groups (P < 0.01). Curcumin had a therapeutic effect, which probably played a role in UC treatment by inhibiting the p38MAPK signaling pathway, thereby reducing the release of TNF-α.
ISSN:1676-5680
1676-5680
DOI:10.4238/2015.april.15.8