Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fer...

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Bibliographic Details
Published in:Cryobiology 2014-12, Vol.69 (3), p.462-466
Main Authors: Kutluyer, Filiz, Kayim, Murathan, Öğretmen, Fatih, Büyükleblebici, Serhat, Tuncer, P. Barbaros
Format: Article
Language:English
Subjects:
Animals
Antioxidant
Antioxidants - metabolism
Cryopreservation - methods
Cryopreservation - veterinary
Female
Fertility - drug effects
Male
Oncorhynchus mykiss
Oncorhynchus mykiss - physiology
Rainbow trout
Sperm Motility - drug effects
Sperm quality
Spermatozoa - cytology
Spermatozoa - drug effects
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Summary:In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250U/l), superoxide dismutase (250U/l), peroxidase (250U/l), oxidized glutathione (1.5mmol/l), reduced glutathione (1.5mmol/l), l-methionine (1.5mmol/l), uric acid (0.25mmol/l), l-ascorbic acid (0.5mmol/l), α-tocopherol (2.0mmol/l), β-carotene (0.5mmol/l) and carnitine (0.5mmol/l). After dilution the semen was aspirated into 0.25ml straws, the straws were placed on the tray, frozen for 10min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, l-methionine, SOD, l-carnitine, α-tocopherol and l-reduced glutathione (p
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2014.10.005