Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program

SHIRPA is a three-stage protocol for the comprehensive assessment of primarily mouse behavior. The first stage consists of high-throughput phenotyping of 33 behavioral observations and 7 metabolic or disease observations. We modified this part of the protocol by integrating new morphologic observati...

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Bibliographic Details
Published in:Mammalian genome 2005-11, Vol.16 (11), p.829-837
Main Authors: Masuya, Hiroshi, Inoue, Maki, Wada, Yumiko, Shimizu, Aya, Nagano, Junko, Kawai, Akiko, Inoue, Ayako, Kagami, Tomoko, Hirayama, Taeko, Yamaga, Ayako, Kaneda, Hideki, Kobayashi, Kimio, Minowa, Osamu, Miura, Ikuo, Gondo, Yoichi, Noda, Tetsuo, Wakana, Shigeharu, Shiroishi, Toshihiko
Format: Article
Language:English
Subjects:
Animals
Behavior
Behavior, Animal
Ethylnitrosourea - pharmacology
Female
Hindlimb - abnormalities
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Mutant Strains - classification
Mice, Mutant Strains - genetics
Mutagenesis
Mutagens
Phenotype
Skin Pigmentation - genetics
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Summary:SHIRPA is a three-stage protocol for the comprehensive assessment of primarily mouse behavior. The first stage consists of high-throughput phenotyping of 33 behavioral observations and 7 metabolic or disease observations. We modified this part of the protocol by integrating new morphologic observations into the initial phenotype assay of behavior and dysmorphology. Behavioral observations assessed by this protocol, now referred to as the "modified-SHIRPA," are compatible with the original "SHIRPA" protocol. Using modified-SHIRPA, we screened dominant phenotypes of more than 10,000 G(1) progeny generated by crossing DBA/2J females with ENU-treated C57BL/6J males. To date, we have obtained 136 hereditary-confirmed mutants that exhibit behavioral and morphologic defects. Some independent mutant lines exhibited similar phenotypes, suggesting that they may represent alleles of the same gene or mutations in the same genetic pathway. They could hold great potential for the unraveling of the molecular mechanisms of certain phenotypes.
ISSN:0938-8990
1432-1777
DOI:10.1007/s00335-005-2430-8