The effect of kisspeptin on the regulation of vascular tone

Kisspeptin has been implicated in cardiovascular control. Eicosanoids play a crucial role in the activation of platelets and the regulation of vascular tone. In the present study, we investigated the effect of kisspeptins on eicosanoid synthesis in platelets and aorta in vitro. Platelets and aorta w...

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Published in:Canadian journal of physiology and pharmacology 2015-09, Vol.93 (9), p.787-791
Main Authors: Mezei, Zsófia, Zamani-Forooshani, Omid, Csabafi, Krisztina, Szikszai, Bence, Papp, Eszter, Ónodi, Ádám, Török, Dóra, Leprán, Ádám, Telegdy, Gyula, Szabó, Gyula
Format: Article
Language:English
Subjects:
6-Ketoprostaglandin F1 alpha - biosynthesis
Animals
Aorta - drug effects
Aorta - metabolism
Arachidonic Acid - metabolism
Biological control systems
Blood platelets
Blood Platelets - drug effects
Blood Platelets - metabolism
Cardiovascular research
Cardiovascular system
Dose-Response Relationship, Drug
Effects
Eicosanoids - biosynthesis
Health aspects
Kisspeptins - pharmacology
Kisspeptins - physiology
Male
Peptides
Physiological aspects
Proteins
Rats
Signal Transduction - drug effects
Signal Transduction - physiology
Thromboxanes - biosynthesis
Vasoconstriction - physiology
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Summary:Kisspeptin has been implicated in cardiovascular control. Eicosanoids play a crucial role in the activation of platelets and the regulation of vascular tone. In the present study, we investigated the effect of kisspeptins on eicosanoid synthesis in platelets and aorta in vitro. Platelets and aorta were isolated from Wistar-Kyoto rats. After preincubation with different doses of kisspeptin, samples were incubated with [1-(14)C]arachidonic acid (0.172 pmol/mL) in tissue culture Medium 199. The amount of labeled eicosanoids was measured with liquid scintillation, after separation with overpressure thin-layer chromatography. Kisspeptin-13 stimulated the thromboxane synthesis. The dose-response curve was bell-shaped and the most effective concentration was 2.5 × 10(-8) mol/L, inducing a 27% increase. Lipoxygenase products of platelets displayed a dose-dependent elevation up to the dose of 5 × 10(-8) mol/L. In the aorta, kisspeptin-13 induced a marked elevation in the production of 6-keto-prostaglandin F1α, the stable metabolite of prostacyclin, and lipoxygenase products. Different effects of kisspeptin on cyclooxygenase and lipoxygenase products indicate that beyond intracellular Ca(2+) mobilization, other signaling pathways might also contribute to its actions. Our data suggest that kisspeptin, through the alteration of eicosanoid synthesis in platelets and aorta, may play a physiologic and (or) pathologic role in the regulation of vascular tone.
ISSN:0008-4212
1205-7541
DOI:10.1139/cjpp-2015-0013