Oligonucleotide and plasmid DNA packaging into polyoma VPI virus-like particles expressed in Escherichia coli

The drug delivery system described here is based on the properties of the capsoid or capsid-like structure resulting from the assembly of polyoma virus capsid protein VPI expressed in Escherichia coli The capsid protein VPI was expressed as a fusion protein with a completely removable N-terminal His...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 1999-02, Vol.29 (1), p.31-43
Main Authors: BRAUN, H, BOLLER, K, LÖWER, J, BERTLING, W. M, ZIMMER, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gene therapy
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Polyomavirus
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Summary:The drug delivery system described here is based on the properties of the capsoid or capsid-like structure resulting from the assembly of polyoma virus capsid protein VPI expressed in Escherichia coli The capsid protein VPI was expressed as a fusion protein with a completely removable N-terminal His sub(6) affinity tag. The pentameric morphology of the recombinant VPI protein was confirmed by electron microscopy after affinity chromatography and factor Xa cleavage under conditions of low ionic strength. The self-assembly of VPI capsoids can be induced from purified VPI pentamers by increasing the ionic strength with (NH sub(4)) sub(2)SO sub(4). These VPI capsoid particles were packed in vitro with antisense oligonucleotides and plasmid DNA. The loading with DNA was pH-dependent. We observed the highest efficiency at pH 5. DNase 1 treatment of particles with encapsidated material showed that 37-55% of the bound oligonucleotides and fragments of 1.5-1.8 kb double-stranded DNA were protected against degradation.
ISSN:0885-4513
1470-8744