Enhanced fluorescence detection of miRNA-16 on a photonic crystal

We report a novel sensing method for fluorescence-labelled microRNAs (miRNAs) spotted on an all-dielectric photonic structure. Such a photonic structure provides an enhanced excitation and a directional beaming of the emitted fluorescence, resulting in a significant improvement of the overall signal...

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Bibliographic Details
Published in:Analyst (London) 2015-08, Vol.14 (16), p.5459-5463
Main Authors: Frascella, F, Ricciardi, S, Pasquardini, L, Potrich, C, Angelini, A, Chiadò, A, Pederzolli, C, De Leo, N, Rivolo, P, Pirri, C. F, Descrovi, E
Format: Article
Language:English
Subjects:
Alignment
Bioassay
Biosensing Techniques - methods
Detection
Emittance
Excitation
Fluorescence
Humans
Limit of Detection
MicroRNAs - analysis
MicroRNAs - genetics
Photonic crystals
Photonics
Photons
Spectrometry, Fluorescence - methods
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Summary:We report a novel sensing method for fluorescence-labelled microRNAs (miRNAs) spotted on an all-dielectric photonic structure. Such a photonic structure provides an enhanced excitation and a directional beaming of the emitted fluorescence, resulting in a significant improvement of the overall signal collected. As a result, the Limit of Detection (LoD) is demonstrated to decrease by a factor of about 50. A compact read-out system allows a wide-field imaging-based detection, with little or no optical alignment issues, which makes this approach particularly interesting for further development for example in microarray-type bioassays. We report a novel sensing method for fluorescence-labelled microRNAs (miRNAs) spotted on an all-dielectric photonic structure.
ISSN:0003-2654
1364-5528
DOI:10.1039/c5an00889a