Visualization of acetaminophen-induced liver injury by time-of-flight secondary ion mass spectrometry

Time-of-flight secondary ion mass spectrometry (MS) provides secondary ion images that reflect distributions of substances with sub-micrometer spatial resolution. To evaluate the use of time-of-flight secondary ion MS to capture subcellular chemical changes in a tissue specimen, we visualized cellul...

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Bibliographic Details
Published in:Analytical biochemistry 2015-11, Vol.488, p.51-58
Main Authors: Murayama, Yohei, Satoh, Shuya, Hashiguchi, Akinori, Yamazaki, Ken, Hashimoto, Hiroyuki, Sakamoto, Michiie
Format: Article
Language:English
Subjects:
Acetaminophen
Acetaminophen - poisoning
Analgesics, Non-Narcotic - poisoning
Animals
Cell Nucleus - chemistry
Cell Nucleus - drug effects
Cell Nucleus - pathology
Chemical and Drug Induced Liver Injury - diagnosis
Chemical and Drug Induced Liver Injury - pathology
Cytoplasm - chemistry
Cytoplasm - drug effects
Cytoplasm - pathology
Diagnostic Imaging - methods
Drug-induced liver injury
Imaging MS
Japan
Liver - chemistry
Liver - drug effects
Liver - pathology
Liver Glycogen - analysis
Mice
Spectrometry, Mass, Secondary Ion
Time-of-flight secondary ion MS
Tissue imaging
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Summary:Time-of-flight secondary ion mass spectrometry (MS) provides secondary ion images that reflect distributions of substances with sub-micrometer spatial resolution. To evaluate the use of time-of-flight secondary ion MS to capture subcellular chemical changes in a tissue specimen, we visualized cellular damage showing a three-zone distribution in mouse liver tissue injured by acetaminophen overdose. First, we selected two types of ion peaks related to the hepatocyte nucleus and cytoplasm using control mouse liver. Acetaminophen-overdosed mouse liver was then classified into three areas using the time-of-flight secondary ion MS image of the two types of peaks, which roughly corresponded to established histopathological features. The ion peaks related to the cytoplasm decreased as the injury became more severe, and their origin was assumed to be mostly glycogen based on comparison with periodic acid–Schiff staining images and reference compound spectra. This indicated that the time-of-flight secondary ion MS image of the acetaminophen-overdosed mouse liver represented the chemical changes mainly corresponding to glycogen depletion on a subcellular scale. In addition, this technique also provided information on lipid species related to the injury. These results suggest that time-of-flight secondary ion MS has potential utility in histopathological applications.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2015.07.005