Mutations in the acetylcholinesterase gene of Bactrocera dorsalis associated with resistance to organophosphorus insecticides

Mutations in the gene encoding the enzyme acetylcholinesterase (AChE) of the oriental fruit fly, Bactrocera dorsalis, associated with resistance to an organophosphorus insecticide have been characterized. Three point mutations producing nonsynonymous changes in the predicted amino acid sequence of t...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2006-05, Vol.36 (5), p.396-402
Main Authors: Hsu, Ju-Chun, Haymer, David S., Wu, Wen-Jer, Feng, Hai-Tung
Format: Article
Language:English
Subjects:
Ace gene
Acetylcholinesterase
Acetylcholinesterase - genetics
Acetylcholinesterase - metabolism
amino acid sequences
Bactrocera
Bactrocera dorsalis
Cholinesterase Inhibitors - pharmacology
DNA Mutational Analysis
Fenitrothion
Fenitrothion - pharmacology
fruit flies
genetic resistance
Insect Proteins - genetics
Insecticide resistance
Insecticide Resistance - genetics
Insecticides - pharmacology
nucleotide sequences
organophosphorus insecticides
Point Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
sequence analysis
Tephritidae
Tephritidae - drug effects
Tephritidae - enzymology
Tephritidae - genetics
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Summary:Mutations in the gene encoding the enzyme acetylcholinesterase (AChE) of the oriental fruit fly, Bactrocera dorsalis, associated with resistance to an organophosphorus insecticide have been characterized. Three point mutations producing nonsynonymous changes in the predicted amino acid sequence of the product of the B. dorsalis ace gene in resistant vs. susceptible flies have been identified. One of these changes is unique to B. dorsalis while the other two occur at sites that are identical to mutations previously described for another Bactrocera species. Although the precise role of the third mutation is not clearly established, the independent origin of two identical alterations in these two species strongly supports the idea proposed previously that molecular changes associated with insecticide resistance in key genes and enzymes such as AChE are largely constrained to a limited number of sites. The results obtained here also suggest that the widespread use of organophosphorus insecticides will likely lead to a predictable acquisition of resistance in wild populations of B. dorsalis as well as other pest species. For surveys of B. dorsalis populations that may develop resistance, diagnostic tests using PCR-RFLP based methods for detecting the presence of all three mutations in individual flies are described.
ISSN:0965-1748
1879-0240
DOI:10.1016/j.ibmb.2006.02.002