Triglyceride-Rich Lipoproteins Modulate the Distribution and Extravasation of Ly6C/Gr1(low) Monocytes

Monocytes are heterogeneous effector cells involved in the maintenance and restoration of tissue integrity. However, their response to hyperlipidemia remains poorly understood. Here, we report that in the presence of elevated levels of triglyceride-rich lipoproteins, induced by administration of pol...

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Bibliographic Details
Published in:Cell reports (Cambridge) 2015-09, Vol.12 (11), p.1802-1815
Main Authors: Saja, Maha F, Baudino, Lucie, Jackson, William D, Cook, H Terence, Malik, Talat H, Fossati-Jimack, Liliane, Ruseva, Marieta, Pickering, Matthew C, Woollard, Kevin J, Botto, Marina
Format: Article
Language:English
Subjects:
Animals
Antigens, Ly - metabolism
Lipoproteins - metabolism
Mice
Mice, Inbred C57BL
Monocytes - metabolism
Triglycerides - metabolism
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Summary:Monocytes are heterogeneous effector cells involved in the maintenance and restoration of tissue integrity. However, their response to hyperlipidemia remains poorly understood. Here, we report that in the presence of elevated levels of triglyceride-rich lipoproteins, induced by administration of poloxamer 407, the blood numbers of non-classical Ly6C/Gr1(low) monocytes drop, while the number of bone marrow progenitors remains similar. We observed an increased crawling and retention of the Gr1(low) monocytes at the endothelial interface and a marked accumulation of CD68(+) macrophages in several organs. Hypertriglyceridemia was accompanied by an increased expression of tissue, and plasma CCL4 and blood Gr1(low) monocyte depletion involved a pertussis-toxin-sensitive receptor axis. Collectively, these findings demonstrate that a triglyceride-rich environment can alter blood monocyte distribution, promoting the extravasation of Gr1(low) cells. The behavior of these cells in response to dyslipidemia highlights the significant impact that high levels of triglyceride-rich lipoproteins may have on innate immune cells.
ISSN:2211-1247
DOI:10.1016/j.celrep.2015.08.020