Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883)

Establishing a cell line from endemic species facilitates the cell biological research of these species in the laboratory. In this study, an epithelium-like cell line RME1 was established from the blastula-stage embryos of the critically endangered cyprinid Honmoroko Gnathopogon caerulescens, which...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2015-09, Vol.51 (8), p.763-768
Main Authors: Higaki, Shogo, Shimada, Manami, Koyama, Yoshie, Fujioka, Yasuhiro, Sakai, Noriyoshi, Takada, Tatsuyuki
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
animal ovaries
Animals
Biomedical and Life Sciences
Cell Biology
Cell Culture
Cell Line - physiology
Culture Media
Cyprinidae - embryology
Developmental Biology
diploidy
Endangered Species
Female
fish
Gene Expression Regulation, Developmental - physiology
genes
Gnathopogon caerulescens
indigenous species
Karyotype
karyotyping
lakes
Life Sciences
Male
nucleotide sequences
Ovary - cytology
Ovary - embryology
polyploidy
Stem Cells
Testis - cytology
Testis - embryology
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Summary:Establishing a cell line from endemic species facilitates the cell biological research of these species in the laboratory. In this study, an epithelium-like cell line RME1 was established from the blastula-stage embryos of the critically endangered cyprinid Honmoroko Gnathopogon caerulescens, which is endemic to ancient Lake Biwa in Japan. To the best of our knowledge, this is the first embryonic cell line from an endangered fish species. This cell line is well adapted to grow at 28°C in the culture medium, which was successfully used for establishing testicular and ovarian cell lines of G. caerulescens, and has displayed stable growth over 60 passages since its initiation in June 2011. Although RME1 did not express the genes detected in blastula-stage embryos, such as oct4, sox2, nanog, and klf4, it showed a high euploidy rate (2n = 50; 67.2%) with normal diploid karyotype morphology, suggesting that RME1 retains the genomic organization of G. caerulescens and can prove to be a useful tool to investigate the unique properties of endangered endemic fishes at cellular level.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-015-9894-y