Starch based polyhydroxybutyrate production in engineered Escherichia coli

Every year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of p...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2015-08, Vol.38 (8), p.1479-1484
Main Authors: Bhatia, Shashi Kant, Shim, Young-Ha, Jeon, Jong-Min, Brigham, Christopher J, Kim, Yong-Hyun, Kim, Hyun-Joong, Seo, Hyung-Min, Lee, Ju-Hee, Kim, Jung-Ho, Yi, Da-Hye, Lee, Yoo Kyung, Yang, Yung-Hun
Format: Article
Language:English
Subjects:
Amylases - biosynthesis
betaine
biodegradability
Biodegradable materials
Biosynthesis
Biotechnology
Carbohydrates
carbon
Chemistry
Chemistry and Materials Science
cost effectiveness
Cupriavidus necator
Cupriavidus necator - enzymology
Cupriavidus necator - genetics
E coli
Environmental Engineering/Biotechnology
Enzymes
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Food Science
genes
Hydroxybutyrates - metabolism
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Metabolic Engineering
Original Paper
plasmids
plastics
Polyesters - metabolism
polyhydroxybutyrate
production costs
Ralstonia
Ralstonia eutropha
Recombinant Proteins - biosynthesis
starch
Starch - metabolism
yeast extract
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Summary:Every year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of polyhydroxyalkanoate by utilizing renewable carbon sources for biosynthesis is an important aspect of commercializing this biodegradable polymer. An Escherichia coli strain that expresses a functional amylase and accumulate polyhydroxybutyrate (PHB), was constructed using different plasmids containing the amylase gene of Panibacillus sp. and PHB synthesis genes from Ralstonia eutropha. This engineered strain can utilize starch as the sole carbon source. The maximum PHB production (1.24 g/L) was obtained with 2 % (w/v) starch in M9 media containing 0.15 % (w/v) yeast extract and 10 mM glycine betaine. The engineered E. coli SKB99 strain can accumulate intracellular PHB up to 57.4 % of cell dry mass.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-015-1390-y