Xylanases from Cellulomonas flavigena : purification and characterization

Three xylanases (Xyl1, Xyl2 and Xyl3) were purified and characterized from the culture supernatant of Cellulomonas flavigena grown on sugar cane bagasse. The enzymes were purified by affinity chromatography and gel filtration and had masses of 63 kDa, 17 kDa and 35 kDa, respectively, as measured by...

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Bibliographic Details
Published in:Biotechnology techniques 1998-09, Vol.12 (9), p.663-666
Main Authors: MONTES HORCASITAS, C, ORTEGA LOPEZ, J, MAGANA PLAZA, I
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cellulomonas flavigena
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
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Summary:Three xylanases (Xyl1, Xyl2 and Xyl3) were purified and characterized from the culture supernatant of Cellulomonas flavigena grown on sugar cane bagasse. The enzymes were purified by affinity chromatography and gel filtration and had masses of 63 kDa, 17 kDa and 35 kDa, respectively, as measured by SDS-PAGE. All enzymes were active against 4-O-methyl-D-glucuronoxylan and xylan but had no cellulase activity with CM-cellulose, an important characteristics in biobleaching processes.
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008852618130