Non-viral transfer of BDNF and uPA stimulates peripheral nerve regeneration

Abstract Peripheral nerves connect brain and spinal cord with the extremities and inner organs, and nerves injury can lead the disability and social exclusion. Growth factors and other natural stimulators of regeneration processes look very promising as future medicines. In our study, we tested the...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2015-08, Vol.74, p.63-70
Main Authors: Karagyaur, M, Dyikanov, D, Makarevich, P, Semina, E, Stambolsky, D, Plekhanova, O, Kalinina, N, Tkachuk, V
Format: Article
Language:English
Subjects:
Animals
BDNF
Brain-Derived Neurotrophic Factor - genetics
Disease Models, Animal
Disease Progression
Gene Transfer Techniques
Genetic Therapy - methods
Genetic Vectors
Internal Medicine
Ischemia - complications
Medical Education
Mice
Mice, Inbred C57BL
Mice, Knockout
Nerve regeneration
Nerve Regeneration - genetics
Peripheral Nerve Injuries - therapy
Plasmids - administration & dosage
Urokinase
Urokinase-Type Plasminogen Activator - genetics
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Summary:Abstract Peripheral nerves connect brain and spinal cord with the extremities and inner organs, and nerves injury can lead the disability and social exclusion. Growth factors and other natural stimulators of regeneration processes look very promising as future medicines. In our study, we tested the influence of genetic constructions that contain genes of brain-derived neurotrophic factor and urokinase plasminogen activator on nerve's structure and function after traumatic and ischemic injuries. Injection of pVax1-hBDNF and pVax1-muPA after traumatic injury led to better restoration of nerve's structure and function compared to similar parameters of control group mice. In ischemic injury model pVax1-hBDNF and pVax1-muPA slowed and reduced the damage progression and stimulated nerve regeneration as well. However, the treatment with pVax1-muPA was less effective after the traumatic injury. As we chose a non-viral method of gene delivery during our study the optimal conditions of plasmid intramuscular delivery were also determined.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2015.07.002