A gold[at]silica core-shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection

The gold nanostar[at]silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, produc...

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Bibliographic Details
Published in:Analytica chimica acta 2014-02, Vol.811, p.76-80
Main Authors: Al-Ogaidi, Israa, Gou, Honglei, Al-kazaz, Abdul, Aguilar, Zoraida, Melconian, Alice, Zheng, Peng, Wu, Nianqiang
Format: Article
Language:English
Subjects:
Assaying
Biosensors
Detection
Enzymes
Glucose
Gold
Nanostructure
Raman scattering
Online Access:Get full text
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Summary:The gold nanostar[at]silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar[at]silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar[at]silica similar to GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25 mu M to 25 mM in the aqueous solution with the limit of detection of 16 mu M. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample.
ISSN:0003-2670
DOI:10.1016/j.aca.2013.12.009