Photodynamic therapy using upconversion nanoparticles prepared by laser ablation in liquid

•Highly crystalline upconversion nanoparticles were prepared by laser ablation in liquid.•Highly transparent near-IR irradiation generated singlet oxygen.•Viability of cancer cells was significantly decreased by near-IR irradiation. Upconversion nanoparticles were prepared by laser ablation in liqui...

Full description

Saved in:
Bibliographic Details
Published in:Applied surface science 2015-09, Vol.348, p.54-59
Main Authors: Ikehata, Tomohiro, Onodera, Yuji, Nunokawa, Takashi, Hirano, Tomohisa, Ogura, Shun-ichiro, Kamachi, Toshiaki, Odawara, Osamu, Wada, Hiroyuki
Format: Article
Language:English
Subjects:
Ablation
Cancer
Cancer therapy
Fluorescence
Laser ablation
Laser ablation in liquid
Liquids
Nanomaterial
Nanoparticles
Photodynamic therapy
Upconversion
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Highly crystalline upconversion nanoparticles were prepared by laser ablation in liquid.•Highly transparent near-IR irradiation generated singlet oxygen.•Viability of cancer cells was significantly decreased by near-IR irradiation. Upconversion nanoparticles were prepared by laser ablation in liquid, and the potential use of the nanoparticles for cancer treatment was investigated. A Nd:YAG/SHG laser (532nm, 13ns, 10Hz) was used for ablation, and the cancer treatment studied was photodynamic therapy (PDT). Morphology and crystallinity of prepared nanoparticles were examined by transmission electron microscopy and X-ray diffraction. Red and green emissions resulting from near-infrared excitation were observed by a fluorescence spectrophotometer. Generation of singlet oxygen was confirmed by a photochemical method using 1,3-diphenylisobenzofuran (DPBF). In vitro experiments using cultivated cancer cells were conducted to investigate PDT effects. Uptake of the photosensitizer by cancer cells and cytotoxicities of cancer cells were also examined. We conclude that the combination of PDT and highly crystalline nanoparticles, which were prepared by laser ablation in liquid, is an effective cancer treatment.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2014.12.097