Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp

The present study was to assess the effect of heavy metal stress on the DNA methylation of a metal‐sensitive plant, Trifolium repens L. and of a metal‐tolerant plant, Cannabis sativa L. The changes in the level of 5‐methylcytosine (5mC) in the root DNA of plants grown on soils contaminated with diff...

Full description

Saved in:
Bibliographic Details
Published in:Physiologia plantarum 2004-07, Vol.121 (3), p.472-480
Main Authors: Aina, Roberta, Sgorbati, Sergio, Santagostino, Angela, Labra, Massimo, Ghiani, Alessandra, Citterio, Sandra
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Cannabis sativa
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on plants and fungi
Fundamental and applied biological sciences. Psychology
Trifolium repens
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study was to assess the effect of heavy metal stress on the DNA methylation of a metal‐sensitive plant, Trifolium repens L. and of a metal‐tolerant plant, Cannabis sativa L. The changes in the level of 5‐methylcytosine (5mC) in the root DNA of plants grown on soils contaminated with different concentrations of Ni2+, Cd2+ and Cr6+ compared with that of untreated plants, were determined by immunolabelling with a monoclonal antibody, using the Slot‐Blot technique. Results showed that DNA of hemp control plants was about three times more methylated than clover DNA, for the same amount of root DNA. Heavy metal treatments induced a global dose‐dependent decrease of 5mC content, both in hemp and clover, ranging from 20 to 40%. Changes in methylation pattern of 5′‐CCGG‐3′ containing sequences were investigated by methylation‐sensitive amplification polymorphism (MSAP) technique. Control plants of the same species showed a very similar pattern, suggesting that, in normal condition, methylation involves precise sites. Heavy metals induced DNA methylation changes mainly related to hypomethylation events. These variations were not randomly directed but involved specific DNA sequences, since the detected polymorphisms were the same in all the plants analysed for each treatment.
ISSN:0031-9317
1399-3054
DOI:10.1111/j.1399-3054.2004.00343.x