Identification of Sex-Specific Markers Reveals Male Heterogametic Sex Determination in Pseudobagrus ussuriensis

Comprehending sex determination mechanism is a first step for developing sex control breeding biotechnologies in fish. Pseudobagrus ussuriensis , one of bagrid catfishes in Bagridae, had been observed to have about threefold size dimorphism between males and females, but its sex determination mechan...

Full description

Saved in:
Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2015-08, Vol.17 (4), p.441-451
Main Authors: Pan, Zheng-Jun, Li, Xi-Yin, Zhou, Feng-Jian, Qiang, Xiao-Gang, Gui, Jian-Fang
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Animals
Aquaculture
Bagridae
Base Sequence
Biomarkers
Biomedical and Life Sciences
Biotechnology
Breeding - methods
Catfish
Catfishes - genetics
Catfishes - physiology
Chromosome Walking
DNA Primers - genetics
Engineering
Female
Females
Fish
Fish stocking
Fisheries
Fishing
Freshwater & Marine Ecology
Genetic Markers - genetics
Genomes
Life Sciences
Male
Males
Microbiology
Molecular Sequence Data
Original Article
Polymorphism
Research centers
Selective breeding
Sequence Alignment
Sequence Analysis, DNA
Sex chromosomes
Sex Chromosomes - genetics
Sex Determination Analysis - methods
Sex Determination Processes - genetics
Studies
Trout
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Comprehending sex determination mechanism is a first step for developing sex control breeding biotechnologies in fish. Pseudobagrus ussuriensis , one of bagrid catfishes in Bagridae, had been observed to have about threefold size dimorphism between males and females, but its sex determination mechanism had been unknown. In this study, we firstly used the amplified fragment length polymorphism (AFLP)-based screening approach to isolate a male-specific DNA fragment and thereby identified a 10,569 bp of male-specific sequence and a 10,365 bp of female-related sequence by genome walking in the bagrid catfish, in which a substantial genetic differentiation with 96.35 % nucleotide identity was revealed between them. Subsequently, a high differentiating region of 650 bp with only 70.26 % nucleotide identity was found from the corresponding two sequences, and three primer pairs of male-specific marker, male and female-shared marker with different length products in male and female genomes, and female-related marker were designed. Significantly, when these markers were used to identify genetic sex of the bagrid catfish, only male individuals was detected to amplify the male-specific marker fragment, and female-related marker was discovered to produce dosage association in females and in males. Our current data provide significant genetic evidence that P. ussuriensis has heterogametic XY sex chromosomes in males and homogametic XX sex chromosomes in females. Therefore, sex determination mechanism of P. ussuriensis is male heterogametic XX/XY system.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-015-9631-2