Production of bacteriocin-like inhibitory substance by Bifidobacterium lactis in skim milk supplemented with additives

Bacteriocins are natural compounds used as food biopreservatives instead of chemical preservatives. Bifidobacterium animalis subsp. lactis (Bifid. lactis) was shown to produce a bacteriocin-like inhibitory substance (BLIS) able to inhibit the growth of Listeria monocytogenes selected as an indicator...

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Bibliographic Details
Published in:Journal of dairy research 2015-08, Vol.82 (3), p.350-355
Main Authors: Martinez, Fabio Andres Castillo, Domínguez, José Manuel, Converti, Attilio, de Souza Oliveira, Ricardo Pinheiro
Format: Article
Language:English
Subjects:
agar
anaerobic conditions
Animals
Bacteria
bacteriocins
Bacteriocins - biosynthesis
Bifidobacterium - growth & development
Bifidobacterium - metabolism
Bifidobacterium animalis
Bifidobacterium animalis subsp. lactis
Bifidobacterium lactis
containers
fermentation
Food Additives
food biopreservatives
Food Microbiology
ingredients
Inulin
Listeria monocytogenes
Listeria monocytogenes - drug effects
Listeria monocytogenes - growth & development
microbial growth
Milk - chemistry
Milk - microbiology
nitrogen
Polysorbates
skim milk
temperature
yeast extract
Yeasts
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Summary:Bacteriocins are natural compounds used as food biopreservatives instead of chemical preservatives. Bifidobacterium animalis subsp. lactis (Bifid. lactis) was shown to produce a bacteriocin-like inhibitory substance (BLIS) able to inhibit the growth of Listeria monocytogenes selected as an indicator microorganism. To enhance this production by the strain Bifid. lactis BL 04, skim milk (SM) was used as a fermentation medium either in the presence or in the absence of yeast extract, Tween 80 or inulin as stimulating additives, and the results in terms of bacterial growth and BLIS production were compared with those obtained in a traditional high cost complex medium such as Man, Rogosa and Sharpe (MRS). To this purpose, all the cultivations were carried out in flasks at 200 rpm under anaerobic conditions ensured by a nitrogen flowrate of 1·0 L/min for 48 h, and BLIS production was quantified by means of a modified agar diffusion assay at low values of both temperature and concentration of List. monocytogenes. Although all these ingredients were shown to exert positive influence on BLIS production in both media, yeast extract and SM were by far the best ingredient and the best medium, respectively, allowing for a BLIS production at the late exponential phase of 2000 AU/ml.
ISSN:0022-0299
1469-7629
1469-7629
DOI:10.1017/S0022029915000163