Evaluation of phenobarbital/β-naphthoflavone as an alternative S9-induction regime to Aroclor 1254 in the rat for use in in vitro genotoxicity assays

A series of bacterial mutation, mammalian cell (L5178Y) gene mutation and in vitro cytogenetic assays were performed to compare the efficacy of using S9 fractions prepared from rats induced with a combination of pheno-barbital (PB) and β-naphthoflavone (βNF), with S9 fractions from rats treated with...

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Bibliographic Details
Published in:Mutagenesis 1995-11, Vol.10 (6), p.517-522
Main Authors: Callander, R. D., Mackay, J. M., Clay, P., Elcombe, C. R., Elliott, B. M.
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Aroclors - toxicity
Benzo(a)pyrene - toxicity
Benzoflavones - administration & dosage
Benzoflavones - toxicity
beta-Naphthoflavone
Biological and medical sciences
Carcinogens - toxicity
Chemical mutagenesis
Chlorodiphenyl (54% Chlorine)
Chromosome Aberrations
Cytochrome P-450 Enzyme System - drug effects
Cytochrome P-450 Enzyme System - metabolism
Dimethylnitrosamine - toxicity
Female
Humans
Liver Extracts - metabolism
Lymphocytes - cytology
Lymphocytes - drug effects
Male
Medical sciences
Mutagenicity Tests - methods
Phenobarbital - administration & dosage
Phenobarbital - toxicity
Rats
Salmonella typhimurium
Salmonella typhimurium - drug effects
Salmonella typhimurium - genetics
Toxicology
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Summary:A series of bacterial mutation, mammalian cell (L5178Y) gene mutation and in vitro cytogenetic assays were performed to compare the efficacy of using S9 fractions prepared from rats induced with a combination of pheno-barbital (PB) and β-naphthoflavone (βNF), with S9 fractions from rats treated with the general enzyme inducer Aroclor 1254. Although some quantitative differences in the magnitudes of the mutagenic/clastogenic effects were observed between the two induction regimes, no qualitative differences were observed. The use of a combined PB/βNF induction regime using oral dosing is therefore considered to be a suitabole substitute for Aroclor 1254.
ISSN:0267-8357
1464-3804
DOI:10.1093/mutage/10.6.517