Combination of exosomes and circulating microRNAs may serve as a promising tumor marker complementary to alpha-fetoprotein for early-stage hepatocellular carcinoma diagnosis in rats

Purpose Due to unsatisfying prognosis of AFP for hepatocellular carcinoma (HCC), we aim to evaluate the prognostic value of combination of exosomes and miRNAs in detecting HCC. Methods HCC was induced with diethylnitrosamine in rats and using a scoring system based on histological examination six di...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2015-10, Vol.141 (10), p.1767-1778
Main Authors: Liu, Wei-hui, Ren, Li-na, Wang, Xing, Wang, Tao, Zhang, Ning, Gao, Yuan, Luo, Hao, Navarro-Alvarez, Nalu, Tang, Li-jun
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - metabolism
Animals
Biomarkers
Biomarkers - blood
Biomarkers - metabolism
Cancer
Cancer Research
Carcinoma, Hepatocellular - blood
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Diagnosis
Exosomes - metabolism
Hematology
Internal Medicine
Liver Cirrhosis - blood
Liver Cirrhosis - metabolism
Liver Cirrhosis - pathology
Liver Neoplasms - blood
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Male
Medicine
Medicine & Public Health
MicroRNAs
MicroRNAs - blood
Molecular biology
Oncology
Original Article – Cancer Research
Rats
Rats, Inbred F344
ROC Curve
Tumors
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Summary:Purpose Due to unsatisfying prognosis of AFP for hepatocellular carcinoma (HCC), we aim to evaluate the prognostic value of combination of exosomes and miRNAs in detecting HCC. Methods HCC was induced with diethylnitrosamine in rats and using a scoring system based on histological examination six different stages (normal liver, degeneration, fibrosis, cirrhosis, early HCC and late HCC) were identified in the development of HCC. The expression levels of AFP, exosomes and miRNAs (miRNA-10b, miRNA-21, miRNA-122 and miRNA-200a) were detected in both tissue and blood samples from those six stages. Receiver operating characteristic (ROC) curve analysis was conducted to evaluate the power of each parameter and their different combinations in diagnosing HCC or cirrhosis. Results A change in the expression of both exosomes and miRNAs was observed during cirrhosis, which in contrast with AFP starts showing up until the early HCC stage. Interestingly, the expressions of exosomes and the selected four miRNAs at early HCC stage obtained more remarkably alterations than the level of AFP ( P  
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-015-1943-0