Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance

Several plant organs contain proteinase inhibitors, which are produced during normal plant development or are induced upon pathogen attack to suppress the enzymatic activity of phytopathogenic microorganisms. In this study, we examined the presence of proteinase inhibitors, specifically trypsin inhi...

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Bibliographic Details
Published in:Genetics and molecular research 2014-11, Vol.13 (4), p.9229-9243
Main Authors: Moulin, M M, Rodrigues, R, Ribeiro, S F F, Gonçalves, L S A, Bento, C S, Sudré, C P, Vasconcelos, I M, Gomes, V M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Capsicum
Capsicum - immunology
Capsicum - virology
Chromatography, Reverse-Phase
Disease Resistance - immunology
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Mosaic Viruses - physiology
Plant Diseases - immunology
Plant Diseases - virology
Plant Extracts - metabolism
Plant Leaves - virology
Trypsin Inhibitors - chemistry
Trypsin Inhibitors - metabolism
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Summary:Several plant organs contain proteinase inhibitors, which are produced during normal plant development or are induced upon pathogen attack to suppress the enzymatic activity of phytopathogenic microorganisms. In this study, we examined the presence of proteinase inhibitors, specifically trypsin inhibitors, in the leaf extract of Capsicum baccatum var. pendulum inoculated with PepYMV (Pepper yellow mosaic virus). Leaf extract from plants with the accession number UENF 1624, which is resistant to PepYMV, was collected at 7 different times (0, 24, 48, 72, 96, 120, and 144 h). Seedlings inoculated with PepYMV and control seedlings were grown in a growth chamber. Protein extract from leaf samples was partially purified by reversed-phase chromatography using a C2/C18 column. Residual trypsin activity was assayed to detect inhibitors followed by Tricine-SDS-PAGE analysis to determine the N-terminal peptide sequence. Based on trypsin inhibitor assays, trypsin inhibitors are likely constitutively synthesized in C. baccatum var. pendulum leaf tissue. These inhibitors are likely a defense mechanism for the C. baccatum var. pendulum- PepYMV pathosystem.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.November.7.10