Differential expression of circulating microRNAs according to severity of colorectal neoplasia

There is a need to develop a colorectal cancer (CRC) screening test that is noninvasive, cost effective, and sensitive enough to detect preneoplastic lesions. This case-control study examined the feasibility of using circulating extracellular microRNAs (miRNAs) to differentiate a spectrum of colorec...

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Published in:	Translational research : the journal of laboratory and clinical medicine 2015-09, Vol.166 (3), p.225-232
Main Authors:	Ho, Gloria Y.F, Jung, Hwa J, Schoen, Robert E, Wang, Tao, Lin, Juan, Williams, Zev, Weissfeld, Joel L, Park, Jung Y, Loudig, Olivier, Suh, Yousin
Format:	Article
Language:	eng
Subjects:	Aged Aged, 80 and over Case-Control Studies Colorectal Neoplasms - blood Colorectal Neoplasms - genetics Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Internal Medicine Male MicroRNAs - blood MicroRNAs - genetics Middle Aged Reproducibility of Results Sequence Analysis, RNA Serum - metabolism
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Summary:	There is a need to develop a colorectal cancer (CRC) screening test that is noninvasive, cost effective, and sensitive enough to detect preneoplastic lesions. This case-control study examined the feasibility of using circulating extracellular microRNAs (miRNAs) to differentiate a spectrum of colorectal neoplasia of various severity and hence for early detection of colorectal neoplasia. Archived serum samples of 10 normal controls and 31 cases, including 10 with nonadvanced adenoma, 10 with advanced adenoma, and 11 with CRC, were profiled for circulating miRNAs using next-generation sequencing. Multiple linear regression, adjusting for age, gender, and smoking status, compared controls and the 3 case groups for levels of 175 miRNAs that met stringent criteria for miRNA sequencing analysis. Of the 175 miRNAs, 106 miRNAs were downregulated according to severity of neoplasia and showed a relative decrease in the expression from controls to nonadvanced adenoma to advanced adenoma to CRC (Ptrend
ISSN:	1931-5244 1878-1810