On-line sensitive lightness measurement of cell mass in Saccharomyces cerevisiae culture

An industrial spectrophotometer was used as a very accurate on-line biomass sensor to investigate fast dynamic changes in yeast culture in the range of 0.5-5 g/l. High sensitive variation in biomass concentration of 0.015 g/l was detected. A fast dynamic response is induced in a steady state continu...

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Bibliographic Details
Published in:Biotechnology techniques 1996, Vol.10 (1), p.19-24
Main Authors: Guillou, V, Queinnec, I, Uribelarrea, J.L, Pareilleux, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
biomass
Biotechnology
cell culture
continuous culture
cultured cells
Fundamental and applied biological sciences. Psychology
measurement
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
reflectance
Saccharomyces cerevisiae
spectrophotometers
spectroscopy
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Summary:An industrial spectrophotometer was used as a very accurate on-line biomass sensor to investigate fast dynamic changes in yeast culture in the range of 0.5-5 g/l. High sensitive variation in biomass concentration of 0.015 g/l was detected. A fast dynamic response is induced in a steady state continuous culture of Saccharomyces cerevisiae by an acetate pulse and biomass concentration profile clearly determined by this sensor.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00161078