Noncationic Rigid and Anisotropic Coiled-Coil Proteins Exhibit Cell-Penetration Activity

Numerous cationic peptides that penetrate cells have been studied intensively as drug delivery system carriers for cellular delivery. However, cationic molecules tend to be cytotoxic and cause inflammation, and their stability in the blood is usually low. We have previously demonstrated that a rigid...

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Bibliographic Details
Published in:Langmuir 2015-08, Vol.31 (30), p.8218-8223
Main Authors: Nakayama, Norihisa, Hagiwara, Kyoji, Ito, Yoshihiro, Ijiro, Kuniharu, Osada, Yoshihito, Sano, Ken-Ichi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anisotropy
Cell Line, Tumor
Cell Survival - drug effects
Cell-Penetrating Peptides - chemistry
Cell-Penetrating Peptides - pharmacology
Dose-Response Relationship, Drug
HeLa Cells
Humans
K562 Cells
Molecular Sequence Data
Structure-Activity Relationship
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Summary:Numerous cationic peptides that penetrate cells have been studied intensively as drug delivery system carriers for cellular delivery. However, cationic molecules tend to be cytotoxic and cause inflammation, and their stability in the blood is usually low. We have previously demonstrated that a rigid and fibrous cationic coiled-coil protein exhibited cell-penetrating ability superior to that of previously reported cell-penetrating peptides. Making use of structural properties, here we describe the cell-penetrating activity of a rigid and fibrous coiled-coil protein with a noncationic surface. A fibrous coiled-coil protein of pI 6.5 penetrated 100% of the cells tested in vitro at a concentration of 500 nM, which is comparable to that of previously reported cell-penetrating peptides. We also investigated the effect of cell-strain dependency and short-term cytotoxicity.
ISSN:0743-7463
1520-5827
DOI:10.1021/acs.langmuir.5b01219