Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Abstract Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2 ) or normoxic (21% O2 ; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hyp...

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Bibliographic Details
Published in:Bone (New York, N.Y.) N.Y.), 2015-09, Vol.78, p.34-45
Main Authors: Lee, Jung-Seok, Park, Jung-Chul, Kim, Tae-Wan, Jung, Byung-Joo, Lee, Youngseok, Shim, Eun-Kyung, Park, Soyon, Choi, Eun-Young, Cho, Kyoo-Sung, Kim, Chang-Sung
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adult
Bone Marrow Cells - cytology
Cell Differentiation
Cell Hypoxia
Cell Proliferation
Cells, Cultured
Chondrocytes - cytology
Chondrogenesis
Collagen - chemistry
Female
Flow Cytometry
Humans
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Male
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
MicroRNAs - metabolism
Middle Aged
Orthopedics
Osteogenesis
Oxygen - chemistry
Regeneration - physiology
Spine - cytology
Stem cell microenvironment interactions
Stem Cells
Tissue regeneration
Wound Healing
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Summary:Abstract Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2 ) or normoxic (21% O2 ; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.
ISSN:8756-3282
1873-2763
DOI:10.1016/j.bone.2015.04.044