MiR-92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN-Mediated PI3K/AKT Pathway

Background MicroRNAs regulate gene expression at the posttranscriptional level and play important roles in tumor development, progression, and metastasis. The aim of this study was to investigate the role of microRNA-92a ( miR-92a ) in metastasis of colorectal cancer (CRC). Methods One hundred fifty...

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Published in:Annals of surgical oncology 2015-08, Vol.22 (8), p.2649-2655
Main Authors: Ke, Tao-Wei, Wei, Po-Li, Yeh, Ken-Tu, Chen, William Tzu-Liang, Cheng, Ya-Wen
Format: Article
Language:English
Subjects:
Aged
Cadherins - genetics
Cadherins - metabolism
Cell Line, Tumor
Cell Movement - genetics
Colorectal Cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Disease-Free Survival
Female
Gene Expression
Gene Knockdown Techniques
Humans
Lymphatic Metastasis
Male
Medicine
Medicine & Public Health
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Oncology
Phosphatidylinositol 3-Kinases - metabolism
Proto-Oncogene Proteins c-akt - metabolism
PTEN Phosphohydrolase - genetics
PTEN Phosphohydrolase - metabolism
Signal Transduction
Surgery
Surgical Oncology
Survival Rate
Up-Regulation
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container_issue 8
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container_title Annals of surgical oncology
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creator Ke, Tao-Wei
Wei, Po-Li
Yeh, Ken-Tu
Chen, William Tzu-Liang
Cheng, Ya-Wen
description Background MicroRNAs regulate gene expression at the posttranscriptional level and play important roles in tumor development, progression, and metastasis. The aim of this study was to investigate the role of microRNA-92a ( miR-92a ) in metastasis of colorectal cancer (CRC). Methods One hundred fifty-eight CRC patients were enrolled. The expression of miR - 92a, PTEN, and E - cadherin was analyzed by real-time PCR. Univariate (Kaplan–Meier) analysis was used to analyze primary outcomes included 5-year overall survival and tumor recurrence. CRC cell model studies were used to analyze the miR-92a -involved CRC metastasis. Results The expression of miR - 92a in tumor tissues was significantly positively correlated with lymph node metastasis in CRC patients ( p  = 0.012). After adjusting for age, sex, and disease differentiation, this correlation remained significant ( p  = 0.01). In addition, there was a negative correlation between levels of miR-92a and the PTEN gene ( p  
doi_str_mv 10.1245/s10434-014-4305-2
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The aim of this study was to investigate the role of microRNA-92a ( miR-92a ) in metastasis of colorectal cancer (CRC). Methods One hundred fifty-eight CRC patients were enrolled. The expression of miR - 92a, PTEN, and E - cadherin was analyzed by real-time PCR. Univariate (Kaplan–Meier) analysis was used to analyze primary outcomes included 5-year overall survival and tumor recurrence. CRC cell model studies were used to analyze the miR-92a -involved CRC metastasis. Results The expression of miR - 92a in tumor tissues was significantly positively correlated with lymph node metastasis in CRC patients ( p  = 0.012). After adjusting for age, sex, and disease differentiation, this correlation remained significant ( p  = 0.01). In addition, there was a negative correlation between levels of miR-92a and the PTEN gene ( p  &lt; 0.0001). No any association of miR - 92a and E-cadherin was found ( p  = 0.128). Patients with high miR - 92a /low PTEN had poorer overall survival and disease-free survival rates than those with high miR - 92a /high PTEN, low miR - 92a /high PTEN, and low miR - 92a /low PTEN. The association of levels of miR - 92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models. Conclusions We suggest that miR-92a is involved in lymph node metastasis of CRC patients through PTEN -regulated PI3K/AKT signaling pathway.</description><identifier>ISSN: 1068-9265</identifier><identifier>EISSN: 1534-4681</identifier><identifier>DOI: 10.1245/s10434-014-4305-2</identifier><identifier>PMID: 25515201</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aged ; Cadherins - genetics ; Cadherins - metabolism ; Cell Line, Tumor ; Cell Movement - genetics ; Colorectal Cancer ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Disease-Free Survival ; Female ; Gene Expression ; Gene Knockdown Techniques ; Humans ; Lymphatic Metastasis ; Male ; Medicine ; Medicine &amp; Public Health ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; Oncology ; Phosphatidylinositol 3-Kinases - metabolism ; Proto-Oncogene Proteins c-akt - metabolism ; PTEN Phosphohydrolase - genetics ; PTEN Phosphohydrolase - metabolism ; Signal Transduction ; Surgery ; Surgical Oncology ; Survival Rate ; Up-Regulation</subject><ispartof>Annals of surgical oncology, 2015-08, Vol.22 (8), p.2649-2655</ispartof><rights>Society of Surgical Oncology 2014</rights><rights>Society of Surgical Oncology 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-aca094f3eecfc1bf120ce65068b443ff7c81426e1f1aac31442540c56085192a3</citedby><cites>FETCH-LOGICAL-c438t-aca094f3eecfc1bf120ce65068b443ff7c81426e1f1aac31442540c56085192a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25515201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ke, Tao-Wei</creatorcontrib><creatorcontrib>Wei, Po-Li</creatorcontrib><creatorcontrib>Yeh, Ken-Tu</creatorcontrib><creatorcontrib>Chen, William Tzu-Liang</creatorcontrib><creatorcontrib>Cheng, Ya-Wen</creatorcontrib><title>MiR-92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN-Mediated PI3K/AKT Pathway</title><title>Annals of surgical oncology</title><addtitle>Ann Surg Oncol</addtitle><addtitle>Ann Surg Oncol</addtitle><description>Background MicroRNAs regulate gene expression at the posttranscriptional level and play important roles in tumor development, progression, and metastasis. The aim of this study was to investigate the role of microRNA-92a ( miR-92a ) in metastasis of colorectal cancer (CRC). Methods One hundred fifty-eight CRC patients were enrolled. The expression of miR - 92a, PTEN, and E - cadherin was analyzed by real-time PCR. Univariate (Kaplan–Meier) analysis was used to analyze primary outcomes included 5-year overall survival and tumor recurrence. CRC cell model studies were used to analyze the miR-92a -involved CRC metastasis. Results The expression of miR - 92a in tumor tissues was significantly positively correlated with lymph node metastasis in CRC patients ( p  = 0.012). After adjusting for age, sex, and disease differentiation, this correlation remained significant ( p  = 0.01). In addition, there was a negative correlation between levels of miR-92a and the PTEN gene ( p  &lt; 0.0001). No any association of miR - 92a and E-cadherin was found ( p  = 0.128). Patients with high miR - 92a /low PTEN had poorer overall survival and disease-free survival rates than those with high miR - 92a /high PTEN, low miR - 92a /high PTEN, and low miR - 92a /low PTEN. The association of levels of miR - 92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models. Conclusions We suggest that miR-92a is involved in lymph node metastasis of CRC patients through PTEN -regulated PI3K/AKT signaling pathway.</description><subject>Aged</subject><subject>Cadherins - genetics</subject><subject>Cadherins - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Colorectal Cancer</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Disease-Free Survival</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine &amp; Public Health</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>Signal Transduction</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Survival Rate</subject><subject>Up-Regulation</subject><issn>1068-9265</issn><issn>1534-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp1kFtLAzEQhYMoXqo_wBcJ-OLL2kwu2_VRlqpFq0XqmxDSdGK3bBtNdpH-e1NaRQQhMCHzzZmTQ8gpsEvgUnUjMClkxkBmUjCV8R1yCCq9yLyA3XRneZFd8VwdkKMY54xBL2H75IArBYozOCSvw-o5IYaOgl_4BiMtsa7pEBsT06ki9Y6WvvYBbWNqWpqlxUDHs-DbtxkdjfuP2RCnlWlwSkcDcd-9vh_TkWlmn2Z1TPacqSOebGuHvNz0x-Vd9vB0OyivHzIrRdFkxhp2JZ1AtM7CxAFnFnOVzE-kFM71bAGS5wgOjLECpORKMqtyVihI3kWHXGx034P_aDE2elFFm_5hlujbqKHHQIiEFwk9_4POfRuWyd2aYrynlGKJgg1lg48xoNPvoVqYsNLA9Dp6vYlep-j1OnrN08zZVrmdLHD6M_GddQL4BoiptXzD8Gv1v6pffAmLEA</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Ke, Tao-Wei</creator><creator>Wei, Po-Li</creator><creator>Yeh, Ken-Tu</creator><creator>Chen, William Tzu-Liang</creator><creator>Cheng, Ya-Wen</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>MiR-92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN-Mediated PI3K/AKT Pathway</title><author>Ke, Tao-Wei ; Wei, Po-Li ; Yeh, Ken-Tu ; Chen, William Tzu-Liang ; Cheng, Ya-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-aca094f3eecfc1bf120ce65068b443ff7c81426e1f1aac31442540c56085192a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Cadherins - genetics</topic><topic>Cadherins - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Colorectal Cancer</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Disease-Free Survival</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine &amp; Public Health</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>Signal Transduction</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Survival Rate</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ke, Tao-Wei</creatorcontrib><creatorcontrib>Wei, Po-Li</creatorcontrib><creatorcontrib>Yeh, Ken-Tu</creatorcontrib><creatorcontrib>Chen, William Tzu-Liang</creatorcontrib><creatorcontrib>Cheng, Ya-Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest - Health &amp; 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The aim of this study was to investigate the role of microRNA-92a ( miR-92a ) in metastasis of colorectal cancer (CRC). Methods One hundred fifty-eight CRC patients were enrolled. The expression of miR - 92a, PTEN, and E - cadherin was analyzed by real-time PCR. Univariate (Kaplan–Meier) analysis was used to analyze primary outcomes included 5-year overall survival and tumor recurrence. CRC cell model studies were used to analyze the miR-92a -involved CRC metastasis. Results The expression of miR - 92a in tumor tissues was significantly positively correlated with lymph node metastasis in CRC patients ( p  = 0.012). After adjusting for age, sex, and disease differentiation, this correlation remained significant ( p  = 0.01). In addition, there was a negative correlation between levels of miR-92a and the PTEN gene ( p  &lt; 0.0001). No any association of miR - 92a and E-cadherin was found ( p  = 0.128). Patients with high miR - 92a /low PTEN had poorer overall survival and disease-free survival rates than those with high miR - 92a /high PTEN, low miR - 92a /high PTEN, and low miR - 92a /low PTEN. The association of levels of miR - 92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models. Conclusions We suggest that miR-92a is involved in lymph node metastasis of CRC patients through PTEN -regulated PI3K/AKT signaling pathway.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25515201</pmid><doi>10.1245/s10434-014-4305-2</doi><tpages>7</tpages></addata></record>
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subjects Aged
Cadherins - genetics
Cadherins - metabolism
Cell Line, Tumor
Cell Movement - genetics
Colorectal Cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Disease-Free Survival
Female
Gene Expression
Gene Knockdown Techniques
Humans
Lymphatic Metastasis
Male
Medicine
Medicine & Public Health
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Oncology
Phosphatidylinositol 3-Kinases - metabolism
Proto-Oncogene Proteins c-akt - metabolism
PTEN Phosphohydrolase - genetics
PTEN Phosphohydrolase - metabolism
Signal Transduction
Surgery
Surgical Oncology
Survival Rate
Up-Regulation
title MiR-92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN-Mediated PI3K/AKT Pathway
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