Imaging the Glycosylation State of Cell Surface Glycoproteins by Two-Photon Fluorescence Lifetime Imaging Microscopy

Glycoproteins in focus: Metabolic labeling of glycans with azido sugars (see picture) in combination with two‐photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both c...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2013-12, Vol.52 (52), p.14045-14049
Main Authors: Belardi, Brian, de la Zerda, Adam, Spiciarich, David R., Maund, Sophia L., Peehl, Donna M., Bertozzi, Carolyn R.
Format: Article
Language:English
Subjects:
Adenocarcinoma - pathology
bioorthogonal chemistry
Biotechnology
Fluorescence
Glycan
Glycoprotein
Glycosylation
Humans
Imaging
Male
Membrane Glycoproteins - analysis
metabolic incorporation
Microscopy
Microscopy, Fluorescence - methods
prostate cancer
Prostatic Neoplasms - pathology
Proteins
Visualization
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Description
Summary:Glycoproteins in focus: Metabolic labeling of glycans with azido sugars (see picture) in combination with two‐photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both cell culture and intact human tissue slices.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201307512