Catching Bubbles: Targeting Ultrasound Microbubbles Using Bioorthogonal Inverse-Electron-Demand Diels-Alder Reactions

Catching bubbles: Tetrazine‐functionalized microbubbles were prepared for use as ultrasound contrast agents, and shown to selectively localize on cells labeled with a TCO‐derivatized antibody against VEGFR2. This capture approach based on labeling and bioorthogonal chemistry was validated in a flow‐...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2014-06, Vol.53 (25), p.6459-6463
Main Authors: Zlitni, Aimen, Janzen, Nancy, Foster, F. Stuart, Valliant, John F.
Format: Article
Language:English
Subjects:
Animal models
Antibodies
Assaying
Bubbles
Cell Line, Tumor
Contrast agents
Contrast media
Contrast Media - chemistry
Diels-Alder reaction
Diels-Alder reactions
Drug Delivery Systems
Humans
Imaging
imaging agents
Marking
Microbubbles
Molecular Structure
tetrazines
Tetrazoles - chemistry
Tumors
Ultrasonic imaging
Ultrasonics
Ultrasound
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Summary:Catching bubbles: Tetrazine‐functionalized microbubbles were prepared for use as ultrasound contrast agents, and shown to selectively localize on cells labeled with a TCO‐derivatized antibody against VEGFR2. This capture approach based on labeling and bioorthogonal chemistry was validated in a flow‐chamber assay and in vivo through ultrasound imaging of VEGFR2‐positive and VEGFR2‐negative murine tumor models. TCO=trans‐cyclooctene.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201402473