A Ratiometric Fluorescent Probe for Rapid Detection of Hydrogen Sulfide in Mitochondria

Quick: An exogenously induced quick increase of the H2S concentration (80 s) in MCF‐7 cells can be visualized by ratiometric imaging using a new probe (CouMC) that can target mitochondria. CouMC was constructed by combining merocyanine and coumarin fluorophores. The selective nucleophilic addition o...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2013-02, Vol.52 (6), p.1688-1691
Main Authors: Chen, Yuncong, Zhu, Chengcheng, Yang, Zhenghao, Chen, Junjie, He, Yafeng, Jiao, Yang, He, Weijiang, Qiu, Lin, Cen, Jiajie, Guo, Zijian
Format: Article
Language:English
Subjects:
Benzopyrans - chemistry
Chemical compounds
Construction
Coumarin
Coumarins - chemistry
Derivatives
Fluorescence
fluorescence imaging
Fluorescent Dyes - chemistry
fluorescent probes
Humans
Hydrogen sulfide
Hydrogen Sulfide - analysis
Hydrogen-Ion Concentration
Imaging
Indoles - chemistry
MCF-7 Cells
Microscopy, Confocal
Mitochondria
Mitochondria - metabolism
ratiometric probes
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Summary:Quick: An exogenously induced quick increase of the H2S concentration (80 s) in MCF‐7 cells can be visualized by ratiometric imaging using a new probe (CouMC) that can target mitochondria. CouMC was constructed by combining merocyanine and coumarin fluorophores. The selective nucleophilic addition of HS− to the merocyanine derivative at neutral pH is crucial for the rapid H2S detection.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201207701