Dissecting Molecular Aspects of Cell Interactions Using Glycodendrimersomes with Programmable Glycan Presentation and Engineered Human Lectins

Glycodendrimersomes with programmable surface display of glycan, together with artificially engineered galectins, were used to understand the physiological significance of human lectins with homodimeric and tandem‐repeat‐type displays. The mode of topological surface presentation and the density of...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2015-03, Vol.54 (13), p.4036-4040
Main Authors: Zhang, Shaodong, Moussodia, Ralph-Olivier, Murzeau, Claire, Sun, Hao-Jan, Klein, Michael L., Vértesy, Sabine, André, Sabine, Roy, René, Gabius, Hans-Joachim, Percec, Virgil
Format: Article
Language:English
Subjects:
adhesion
agglutination
Binding sites
Binding Sites - drug effects
Carbohydrate Conformation
Carbohydrates
Carbohydrates - chemistry
Cell Line, Tumor
Cell Membrane - chemistry
Cells - drug effects
Computer programs
Concanavalin A - chemistry
Cross-Linking Reagents
Crosslinking
dendrimers
Dendrimers - chemistry
Density
Fluorescent Dyes
Galectins - chemical synthesis
Galectins - chemistry
Glycan
Guidelines
Human
Humans
lectin
Lectins
Models, Molecular
Surface Properties
Tandem Repeat Sequences
vesicles
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Summary:Glycodendrimersomes with programmable surface display of glycan, together with artificially engineered galectins, were used to understand the physiological significance of human lectins with homodimeric and tandem‐repeat‐type displays. The mode of topological surface presentation and the density of glycan affected vesicle aggregation mediated by multivalent carbohydrate–protein interactions. The cross‐linking capacity of homodimeric lectins was enhanced by covalent connection of the two carbohydrate‐binding sites. These findings highlight the value of glycodendrimersomes as versatile cell membrane mimetics, and assays provide diagnostic tools for protein functionality. This work also provides guidelines for the design of cell separators, bioactive matrices, bioeffectors, and other biomedical applications. A combination of supramolecular glycodendrimersomes with programmed glycan presentation and engineering of human galectin‐1 provides answers to fundamental questions on structure–activity relationships in cell interactions and guidelines for the design of new tools for biomedical applications.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201410882