Hapten synthesis and development of ELISAs for detection of endosulfan in water and soil

Two enzyme immunoassays, a laboratory assay based on microwell plates and a field test based on the use of small polystyrene tubes, have been developed for the detection of endosulfan (1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-en-2,3-ylenedime thyl sulfite) residues in water and soil. To raise anti...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1995-06, Vol.43 (6), p.1730-1739
Main Authors: Lee, Nanju, Skerritt, John H, McAdam, David P
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
ANTIBODIES
ANTICORPS
ANTICUERPOS
Biological and medical sciences
CHEMICAL REACTIONS
Economic plant physiology
ELISA
ENDOSULFAN
ENDOSULFAN SULFATE
Fundamental and applied biological sciences. Psychology
Growth and development
HAPTENE
HAPTENOS
HAPTENS
INSECTICIDAS
INSECTICIDE
INSECTICIDES
METABOLITE
METABOLITES
METABOLITOS
MICROWELL ASSAY
POLLUTED SOIL
POLLUTED WATER
POLLUTION DE L'EAU
POLUCION DEL AGUA
REACCIONES QUIMICAS
REACTION CHIMIQUE
RESIDU
RESIDUES
RESIDUOS
SOL POLLUE
SUELO CONTAMINADO
TEST ELISA
TUBE ASSAY
WATER POLLUTION
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Summary:Two enzyme immunoassays, a laboratory assay based on microwell plates and a field test based on the use of small polystyrene tubes, have been developed for the detection of endosulfan (1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-en-2,3-ylenedime thyl sulfite) residues in water and soil. To raise antibodies that are sensitive and selective for the toxic forms of endosulfan, three haptens were prepared. One hapten was prepared by derivatization of endosulfan diol 1,4,5,6,7,7-hexachlor- 2,3-bis(hydroxymethyl)norborn-5-ene), while the others used derivatives of a rigid five-membered ring adjacent to the bridged hexachlorocydopentadiene (cyclodiene) ring. Different hapten combinations were used for immunogen and reporter enzyme conjugate in both the microwell and field assays. The optimized assays have detection limits of about 0.2 ppb endosulfan and detect in the range 0.2-10 ppb (0.2-20 ppb for field assay, without sample dilution). Water samples can be analyzed directly without solvent extraction or concentration, while soil samples are simply extracted with 90% methanol. The tests detect endosulfan sulfate (1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-en- 2,3-ylenedimethyl sulfate) with sensitivity similar to that for endosulfan but are 4-10 times less sensitive to endosulfan diol, and therefore the assays can potentially determine toxic compounds of endosulfan (endosulfan and endosulfan sulfate) from the total endosulfan residues present in the environment
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00054a060