Measurement of a Urinary Marker (8-hydroxydeoxy­Guanosine, 8-OHdG) of DNA Oxidative Stress in Epidemiological Surveys: A Pilot Study

Background 8-Hydroxydeoxyguanosine (8-OHdG) is a commonly used marker of DNA oxidative stress in epidemiological studies. The aim of this study was to establish whether the urinary concentration of 8-OHdG varies during the first part of the day, when clinical tests are usually performed, and whether...

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Bibliographic Details
Published in:The International journal of biological markers 2015-07, Vol.30 (3), p.341-345
Main Authors: Zanolin, M. Elisabetta, Girardi, Paolo, Degan, Paolo, Rava, Marta, Olivieri, Mario, Di Gennaro, Gianfranco, Nicolis, Morena, De Marco, Roberto
Format: Article
Language:English
Subjects:
Adult
Biomarkers
Body Mass Index
Circadian Rhythm
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - urine
Diet
DNA Damage
DNA Repair
Female
Fruit
Habits
Humans
Male
Middle Aged
Motor Activity
Oxidative Stress
Pilot Projects
Smoking - epidemiology
Smoking - urine
Surveys and Questionnaires
Vegetables
Young Adult
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Summary:Background 8-Hydroxydeoxyguanosine (8-OHdG) is a commonly used marker of DNA oxidative stress in epidemiological studies. The aim of this study was to establish whether the urinary concentration of 8-OHdG varies during the first part of the day, when clinical tests are usually performed, and whether it can therefore be measured without bias in spot urine samples. Material and methods Spot urine samples were collected using a convenience sample. A linear mixed-effects model for repeated measurements was used to analyze 8-OHdG levels. Results A significant increasing trend in time in the 8-OHdG concentration was found among smokers, but not in the case of nonsmokers. Conclusions In epidemiological studies on oxidative stress, all participants should collect their early morning urine specimens – before their first cigarette if they are smokers – to gather information on individual background oxidation levels.
ISSN:1724-6008
1724-6008
DOI:10.5301/jbm.5000129