A molecular method for S-allele identification in apple based on allele-specific PCR

cDNA sequences corresponding to two self-incompatibility alleles (S-alleles) of the apple cv 'Golden Delicious' have previously been described, and now the identification of three additional S-allele cDNAs of apple is reported, one of which was isolated from a pistil cDNA library of cv �...

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Bibliographic Details
Published in:Theoretical and applied genetics 1995-09, Vol.91 (4), p.691-698
Main Authors: Janssens, G.A, Goderis, I.J, Broekaert, W.F, Broothaerts, W. (Katholieke Univ. Leuven (Belgium). Lab. of Genetics)
Format: Article
Language:English
Subjects:
AUTOESTERILIDAD
AUTOSTERILITE
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
GENE
GENES
GENETICA MOLECULAR
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE MOLECULAIRE
MALUS PUMILA
Pteridophyta, spermatophyta
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Vegetals
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Summary:cDNA sequences corresponding to two self-incompatibility alleles (S-alleles) of the apple cv 'Golden Delicious' have previously been described, and now the identification of three additional S-allele cDNAs of apple is reported, one of which was isolated from a pistil cDNA library of cv 'Idared' and two of which were obtained by reverse transcription-PCR (RT-PCR) on pistil RNA of cv 'Queen's Cox'. A comparison of the deduced amino acid sequences of these five S-allele cDNAs revealed an average homology of 69%. Based on the nucleotide sequences of these S-allele cDNAs, a molecular technique was developed for the diagnostic identification of the five different S-alleles in apple cultivars. The method used consists of allele-specific PCR amplification of genomic DNA followed by digestion of the amplification product with an allele-specific restriction endonuclease. Analysis of a number of apple cultivars with known S-phenotype consistently showed coincidence of phenotypic and direct molecular data of the S-allele constitution of the cultivars. It is concluded that the S-allele identification approach reported here provides a rapid and useful method to determine the S-genotype of apple cultivars.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00223298