Interference Reduction in Glucose Detection by Redox Potential Tuning: New Glucose Meter Development

A new glucose meter was developed employing a novel disposable glucose sensor strip comprising a nicotinamide adenine dinucleotide-glucose dehydrogenase (NAD-GDH) and a mixture of Fe compounds as a mediator. An iron complex, 5-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron(III) chlo...

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Bibliographic Details
Published in:Analytical Sciences 2015/07/10, Vol.31(7), pp.705-710
Main Authors: CHO, Seong Je, CHO, Chul-Ho, KIM, Kwang Bok, LEE, Min-Hyoung, KIM, Jae Hong, LEE, Suho, CHO, Jaegeol, JUNG, Suntae, KIM, Dong-Min, SHIM, Yoon-Bo
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biosensing Techniques - methods
Chemistry
Dimethyl Sulfoxide - chemistry
Electrochemistry
Electrodes
Ferricyanides - chemistry
Glucose - analysis
Glucose - chemistry
Glucose 1-Dehydrogenase - metabolism
Glucose sensor
Humans
mixed Fe-complex mediator
NAD - metabolism
NAD-GDH
Oxidation-Reduction
potential tuning
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Summary:A new glucose meter was developed employing a novel disposable glucose sensor strip comprising a nicotinamide adenine dinucleotide-glucose dehydrogenase (NAD-GDH) and a mixture of Fe compounds as a mediator. An iron complex, 5-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron(III) chloride (Fe-PhenTPy), was synthesized as a new mediator for the NAD-GDH system. Due to the high oxidation potential of the mediator, the detection potential was tuned to be more closely fitted toward the enzyme reaction potential, less than 400 mV (vs. Ag/AgCl), by mixing with an additional iron mediator. The impedance spectrometry for the enzyme sensor containing the mixed mediators showed an enhanced charge transfer property. In addition, a new cartridge-type glucose meter was manufactured using effective aligned-electrodes, which showed an enhanced response compared with conventional electrode alignment. The proposed glucose sensor resulted in a wide dynamic range in the concentration range of 30 – 500 mg dL−1 with a reduced interference effect and a good sensitivity of 0.57 μA mM−1.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.31.705