Cloning and Sequencing of a Hemagglutinin Component of the Botulinum Neurotoxin Complex encoded by Clostridium botulinum Types A and B
Degenerate oligonucleotide primers designed to the N-terminal amino acid sequence of proteins purified from the botulinum neurotoxin complex were used to amplify DNA fragments using PCR from genomic DNA of Clostridium botulinum types A: NCTC 7272, and B: NCTC 7273 (proteolytic strain) and Eklund 17B...
Saved in:
| Published in: | Systematic and applied microbiology 1994, Vol.17 (3), p.306-312 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Degenerate oligonucleotide primers designed to the N-terminal amino acid sequence of proteins purified from the botulinum neurotoxin complex were used to amplify DNA fragments using PCR from genomic DNA of
Clostridium botulinum types A: NCTC 7272, and B: NCTC 7273 (proteolytic strain) and Eklund 17B (non-proteolytic strain). A fragment of ~ 1.9 kb was amplified using template DNA from each strain, which was subsequently cloned in
E. coli and the sequences determined. Two open reading frames (orfs) were discovered: one corresponding to a protein ~ 33.7 kD which shows between 36 and 41% identity with the major hemagglutinin component (HA-33) of the botulinum progenitor complex encoded by C.
botulinum type C. The second orf, encoding a protein of ~ 21.7 kD, P-21, of 178 amino acids (179 in strain NCTC 7273) has ~ 93% identity between the type A and two type B strains, but is absent from type C. |
|---|---|
| ISSN: | 0723-2020 1618-0984 |