Genetic polymorphism of 13 non-CODIS STR loci in three national populations from China

The aim of this study was to investigate a 13 non‐CODIS STR loci database using three national populations from China. A new multiplex PCR system that simultaneously amplified 13 loci in the same PCR reaction was developed. This multiplex system included the 13 STR markers (D3S2402, D3S2452, D3S1766...

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Bibliographic Details
Published in:Electrophoresis 2014-12, Vol.35 (23), p.3395-3401
Main Authors: Liu, Qiu-Ling, Huang, Kai-Kai, Wu, Ye-Da, Zhao, Hu, Li, Cheng-tao, Lu, De-Jian
Format: Article
Language:English
Subjects:
Asian Continental Ancestry Group - genetics
China
Databases, Genetic
Female
Genetic Markers - genetics
Genetic polymorphism
Genetics
Haplotype
Humans
Linkage
Loci
Male
Markers
Microsatellite Repeats
Multiplex PCR
Multiplex Polymerase Chain Reaction - methods
Multiplexing
Mutation - genetics
Mutations
Polymorphism, Genetic
Populations
STR
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Summary:The aim of this study was to investigate a 13 non‐CODIS STR loci database using three national populations from China. A new multiplex PCR system that simultaneously amplified 13 loci in the same PCR reaction was developed. This multiplex system included the 13 STR markers (D3S2402, D3S2452, D3S1766, D3S4554, D3S2388, D3S3051, D3S3053, D4S2364, D4S2404, AC001348A, AC001348B, D17S975, and D17S1294), which were successfully analyzed by using 441 DNA samples from three national populations in China (154 Mongol, 177 Kazakh, and 110 Uigur). Allele frequencies and mutation rates of the 13 non‐CODIS STR loci were investigated. A total of 4–10 alleles at each locus were observed and altogether 84, 88, and 87 alleles for the all selected loci were found in the Mongol, Kazakh, and Uigur, respectively. Eight mutations were detected from the 13 selected loci in 9880 meioses in kinship cases. These results indicate that this multiplex system may provide significant polymorphic information for kinship testing and relationship investigations.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201400290