Electrochemical Biosensing Platform Using Hydrogel Prepared from Ferrocene Modified Amino Acid as Highly Efficient Immobilization Matrix

To increase the loading of glucose oxidase (GOx) and simplify glucose biosensor fabrication, hydrogel prepared from ferrocene (Fc) modified amino acid phenylalanine (Phe, F) was utilized for the incorporation of GOx. The synthesized hydrogel displays good biocompatibility and contains a significant...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2014-01, Vol.86 (2), p.973-976
Main Authors: Qu, Fengli, Zhang, Yi, Rasooly, Avraham, Yang, Minghui
Format: Article
Language:English
Subjects:
Amino acids
Biochemistry
Biocompatibility
Biosensing Techniques - methods
Biosensors
Blood Glucose - analysis
Displays
Electrochemical Techniques
Electrodes
Electron Transport
Enzymes
Enzymes, Immobilized
Equipment Design
Ferrocenes
Ferrous Compounds - chemistry
Glucose
Glucose Oxidase - chemistry
Humans
Hydrogels
Metallocenes
Microscopy, Electron, Scanning
Nanofibers - chemistry
Nanofibers - ultrastructure
Phenylalanine - chemistry
Scanning electron microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To increase the loading of glucose oxidase (GOx) and simplify glucose biosensor fabrication, hydrogel prepared from ferrocene (Fc) modified amino acid phenylalanine (Phe, F) was utilized for the incorporation of GOx. The synthesized hydrogel displays good biocompatibility and contains a significant number of Fc moieties, which can be considered as an ideal matrix to immobilize enzymes for the preparation of mediator-based biosensors. The hydrogel was studied by scanning electron microscopy, which indicated that it was composed of nanofibers with a diameter of around 50–100 nm and length extended to 1 mm. With the addition of GOx into the hydrogel and by directly dropping the resulting biocomposite onto the electrode surface, a glucose biosensor, that displays good performance due to improved enzyme loading and efficient electron transfer, can be simply constructed. The favorable network structure and good biocompatibility of the hydrogel could effectively avoid enzyme leakage and maintain the bioactivity of the enzymes, which resulted in good stability of the biosensor. The biosensor was utilized for the detection of glucose in blood samples with results comparable to those obtained from the hospital. The hydrogel as a functional component of an amperometric biosensor has implications for future development of biosensors and for clinical applications.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac403478z