Label-free detection of multidrug resistance in K562 cells through isolated 3D-electrode dielectrophoresis

Dielectrophoresis (DEP), a technique used to separate particles based on different sizes and/or dielectric properties under nonuniform electric field, is a promising method to be applied in label‐free, rapid, and effective cell manipulation and separation. In this study, a microelectromechanical sys...

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Bibliographic Details
Published in:Electrophoresis 2015-05, Vol.36 (9-10), p.1149-1157
Main Authors: Demircan, Yağmur, Koyuncuoğlu, Aziz, Erdem, Murat, Özgür, Ebru, Gündüz, Ufuk, Külah, Haluk
Format: Article
Language:English
Subjects:
3D-electrode
Arrays
Cell Separation - instrumentation
Cell Separation - methods
Channels
Computer Simulation
Devices
Dielectrophoresis
Drug Resistance, Multiple
Electric fields
Electrodes
Electrophoresis - instrumentation
Electrophoresis - methods
Humans
Isolated electrode dielectrophoresis
K562 Cells
Leukemia
Leukemias
Limit of Detection
Multidrug resistance
Obstacles
Parylene
Trapping
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Summary:Dielectrophoresis (DEP), a technique used to separate particles based on different sizes and/or dielectric properties under nonuniform electric field, is a promising method to be applied in label‐free, rapid, and effective cell manipulation and separation. In this study, a microelectromechanical systems‐based, isolated 3D‐electrode DEP device has been designed and implemented for the label‐free detection of multidrug resistance in K562 leukemia cells, based on the differences in their cytoplasmic conductivities. Cells were hydrodynamically focused to the 3D‐electrode arrays, placed on the side walls of the microchannel, through V‐shaped parylene‐C obstacles. 3D‐electrodes extruded along the z‐direction provide uniformly distributed DEP force through channel depth. Cell suspension containing resistant and sensitive cancer cells with 1:100 ratio was continuously flown through the channel at a rate of 10 μL/min. Detection was realized at 48.64 MHz, the cross‐over frequency of sensitive K562 cells, at which sensitive cells flow with the fluid, while the resistant ones are trapped by positive DEP force. Device can be operated at considerably low voltages (
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201400391