Establishment of a Trimodality Analytical Platform for Tracing, Imaging and Quantification of Gold Nanoparticles in Animals by Radiotracer Techniques

This study aims to establish a 198Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatil...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-01, Vol.87 (1), p.601-608
Main Authors: Chen, Chien-Hung, Lin, Fong-Sian, Liao, Wei-Neng, Liang, Sanching L, Chen, Min-Hua, Chen, Yo-Wen, Lin, Wan-Yu, Hsu, Ming-Hua, Wang, Mei-Ya, Peir, Jinn-Jer, Chou, Fong-In, Chen, Ching-Ya, Chen, Sih-Yu, Huang, Su-Chin, Yang, Mo-Hsiung, Hueng, Dueng-Yuan, Hwu, Yeukuang, Yang, Chung-Shi, Chen, Jen-Kun
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Chemical compounds
Diagnostic Imaging - methods
Gold
Gold - chemistry
Half-Life
Imaging
Male
Mathematical analysis
Metal Nanoparticles - chemistry
Mice
Mice, Inbred ICR
Nanoparticles
Organs
Particle Size
Platforms
Polyethylene glycol
Polyethylene Glycols - chemistry
Radiation
Radioactive Tracers
Radionuclide Imaging
Tissue Distribution
Visualization
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Summary:This study aims to establish a 198Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. 198Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, 198Au can be used for bioanalytical purposes. The 198Au-tagged radioactive gold nanoparticles (198Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the 197Au­(n,γ)198Au reaction and subsequently the 198Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated 198Au-GNPs. The 198Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of 198Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The 198Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95% recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of 198Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from 198Au, enabled visualizing the heterogeneous biodisposition of 198Au-GNPs in different microenvironments and tissues. In this study, the 198Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac503260f