Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by newly isolated Agrobacterium sp. SH-1 and GW-014 from structurally unrelated single carbon substrates

Various isolates from activated sludge and soil samples were investigated for their ability to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), from structurally unrelated single carbon substrates. Isolated Gram-negative bacteria, Agrobacterium sp. SH-1 and GW-014, could synth...

Full description

Saved in:
Bibliographic Details
Published in:Journal of fermentation and bioengineering 1995-01, Vol.79 (4), p.328-334
Main Authors: Lee, Eun Yeol, Kang, Sang Hyeon, Choi, Cha Yong
Format: Article
Language:English
Subjects:
AGROBACTERIUM
Agrobacterium sp
Bioconversions. Hemisynthesis
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Biotechnology
BUTIRATOS (SALES)
BUTYRATE (SEL)
BUTYRATES (SALTS)
COMPOSE HYDROXYLE
COMPUESTOS DE HIDROXILO
Fundamental and applied biological sciences. Psychology
GLUCOSA
GLUCOSE
HYDROXY COMPOUNDS
Methods. Procedures. Technologies
POLIMEROS
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
polyhydroxyalkanoate
POLYMERE
POLYMERS
SACCHAROSE
single carbon substrate
SUCROSA
SUCROSE
XILOSA
XYLOSE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various isolates from activated sludge and soil samples were investigated for their ability to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), from structurally unrelated single carbon substrates. Isolated Gram-negative bacteria, Agrobacterium sp. SH-1 and GW-014, could synthesize P(3HB-co-3HV) with 3HV monomer mole percent in the range from 1.3 to 11.4 from single carbon substrate, such as glucose, xylose or sucrose. Terpolyesters of 3HB, 3HV and 4-hydroxybutyrate (4HB) were also accumulated by Agrobacterium sp. SH-1 and GW-014 in a nitrogen-free production stage with various hydroxyalkanoate substrates. The structure of P(3HB-co-3HV) synthesized from glucose was confirmed by gas chromatographicmass spectrometric (GC-MS) analysis, and its molecular weight, polydispersity index and melting temperature were determined to be 620000, 1.2 and 178°C, respectively. Total polymer content up to 78% (w/w) of dry cell weight and 3HV mole percent up to 50% could be achieved by fed-batch production from glucose and propionate.
ISSN:0922-338X
DOI:10.1016/0922-338X(95)93990-2